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Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf

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Abstract

Kalopanax pictus is a deciduous tree used in traditional medicine. The leaves have been consumed as a vegetable in Korea. In this study, we examined the antioxidant and anti-inflammatory capacity of the extracts from K. pictus leaves in vitro using hydroxyl radical scavenging assay, DNA nicking assay, metal chelating assay, reducing power assay, DPPH free radical scavenging assay, and nitric oxide inhibition assay. The effect of the hot water extract was stronger than that of the methanolic and ethanolic extracts in all of the assays. Moreover, the total phenolic contents in the extracts were measured by spectrophotometry. Meanwhile, a regression analysis revealed a correlation coefficient between the content of phenolics and antioxidant activity. Overall, our data suggest that K. pictus leaves possess significant antioxidant properties.

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Abbreviations

BHA:

butylated hydroxyanisole

BHT:

butylated hydroxytoluene

DPPH:

2,2-diphenyl-l-pricylhydrazyl radical

EC50 :

the effective concentration at which the absorbance was 0.5

FBS:

fetal bovine serum

Ferrozine:

(4-phenyl-sulfonic acid)-1,2,4-triazine

IC50 :

half-inhibition concentration

NO:

nitrite oxide

•OH:

hydroxyl radical

ROS:

reactive oxygen species

TCA:

3-trichloroacetic acid

TBHQ:

tert-butylhydroquinone

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Authors and Affiliations

  1. School of Biotechnology, Kangwon National University, 200-701, Chuncheon, Republic of Korea

    Weicheng Hu, Seong-Il Heo & Myeong-Hyeon Wang

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  1. Weicheng Hu
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  2. Seong-Il Heo
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  3. Myeong-Hyeon Wang
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Correspondence to Myeong-Hyeon Wang.

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Hu, W., Heo, SI. & Wang, MH. Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf. J. Korean Soc. Appl. Biol. Chem. 52, 360–366 (2009). https://doi.org/10.3839/jksabc.2009.064

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  • DOI: https://doi.org/10.3839/jksabc.2009.064

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