Abstract
The adjuvant effect of two types of water extracts of Cudrania tricuspidata (CT), cold water extract (CT-CW) and hot water extract (CT-HW), on induction of humoral and cellular immune responses against keyhole limpet hemocyanine (KLH) was examined. When mice were immunized subcutaneously (s.c.) twice at 2-week interval with KLH (20 μg/mouse) admixed with or without 50 mg/mouse of CT-CW or CT-HW, mice immunized with KLH+CT-CW and KLH+CT-HW showed significantly higher antibody titers against KLH than those immunized with KLH alone. Increase of the number of KLH-specific antibody producing B lymphocytes was observed in mice immunized admixed with CT-CW and CT-HW. The assay for determining isotypes of antibodies showed that both CT-CW and CT-HW augmented KLH-specific antibody titers of IgG1, IgG2a and IgG2b. In an in vitro analysis of T lymphocyte proliferation to KLH on week 4, the splenocytes of both mice treated with KLH+CT-CW and KLH+CT-HW showed a significantly higher proliferating activity than those treated with KLH alone. And the culture supernatants of the cell cultures from both mice immunized admixed with CT-CW and CT-HW showed a higher level of KLH-specific IL-2 production. Furthermore, mice immunized twice with KLH+CT-CW and KLH+CT-HW and followed by intrafootpad (i.f.) injection of KLH (20 μg/site) 7 weeks after the primary immunization induced a higher delayed-type hypersensitivity (DTH) reaction than mice treated with KLH alone. These results suggest that CT possesses a potent immunoadjuvant activity to enhance antigen-specific immune responses.
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Abbreviations
- BSA:
-
bovine serum albumin
- CT:
-
Cudrania tricuspidata
- CT-CW:
-
cold-water extract of Cudrania tricuspidata
- CT-HW:
-
hot-water extract of Cudrania tricuspidata
- DTH:
-
delayed-type hypersensitivity
- ELISA:
-
enzyme-linked immunosorbent assay
- ELISPOT:
-
enzyme-linked immunospot
- KLH:
-
keyhole limpet hemocyanine
- SFC:
-
spot forming cells
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Lee, K.B., Song, KS., Moon, E.H. et al. Adjuvant activity of cudrania tricuspidata water extracts to enhance antigen specific humoral and cellular immune responses. J. Korean Soc. Appl. Biol. Chem. 52, 234–240 (2009). https://doi.org/10.3839/jksabc.2009.042
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DOI: https://doi.org/10.3839/jksabc.2009.042