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Antioxidative and anti-inflammatory activities of Litsea japonica leaves

  • Weon-Jong Yoon
  • Se Chan Kang
  • Young-Min Ham
  • Kil-Nam Kim
  • Weon Hyuk Yang
  • Hee-Jung Kim
  • Soo-Yeong Park
  • Yong-Hwan Jung
Bioactive Material Article

Abstract

We investigated the antioxidative and anti-inflammatory properties of an 80% ethanol extract and solvent fractions of Litsea japonica leaves. The extract and the CH2Cl2, EtOAc, and BuOH fractions showed antioxidant potential, based on radical-scavenging activity. In LPS-stimulated RAW 264.7 cells, the CH2Cl2 fractions inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production. Additionally, the CH2Cl2 fractions inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. These results show that the CH2Cl2 fractions of L. japonica strongly inhibited the production of NO and PGE2 and the induction of iNOS and COX-2 in LPS-stimulated cells. Thus, we propose that CH2Cl2 fractions may inhibit NO and PGE2 production primarily through the regulation of iNOS and COX-2 gene expression. This study suggests the L. japonica may be a natural source of effective antioxidant and anti-inflammatory agents.

Key words

anti-inflammatory antioxidant cyclooxygenase-2 Litsea japonica nitric oxide prostaglandin E2 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Weon-Jong Yoon
    • 1
  • Se Chan Kang
    • 2
  • Young-Min Ham
    • 1
  • Kil-Nam Kim
    • 1
  • Weon Hyuk Yang
    • 1
  • Hee-Jung Kim
    • 1
  • Soo-Yeong Park
    • 1
  • Yong-Hwan Jung
    • 1
  1. 1.Jeju Biodiversity Research InstituteJeju HiDISeogwipoRepublic of Korea
  2. 2.Department of Natural Medicine ResourcesSemyung UniversityJecheonRepublic of Korea

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