Abstract
Special difficulties are encountered when attempting to provide intracranial cannulation of small animals that have floating or fragile skull bones. The technique described was developed for mice but seems applicable to other species. The cannula system is inexpensive and easy to use. A wound clip anchor seems superior to either skull screws or bonded acrylic, producing less damage to the brain and no necrosis of bone or tissue on the wound margin. Patency data up to 25 days is presented, and the system is as efficacious as other techniques.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Crane, L. A., &Glick, S. D. Simple cannula for repeated intracranial drug administration in rats.Pharmacology, Biochemistry and Behavior, 1979,10, 799–800.
Kokkinidis, L., RAffler, L., &Anisman, H. Simple and compact cannula system for mice.Pharmacology, Biochemistry and Behavior, 1977,6, 595–597.
Laird, H. E. II,Hermansen, J. E., &Huxtable, R. J. An electrode-cannula unit for intracerebral electrical stimulation, EEG recording and drug administration in small animals.Pharmacology, Biochemistry and Behavior, 1979,10, 429–431.
Lehmann, A.Atlas stereotaxique du cerveau de la souris. Paris: Centre National de la Recherche Scientifique, 1974.
Routtenberg, A. Intracranial chemical injection and behavior: Acritical review.Behavioral Biology, 1972,7, 601–641.
Villiger, J. W., &Chute, D. L. Papaverine facilitates passive avoidance in mice.Society for Neuroscience Abstracts, 1979,5, 326.
Author information
Authors and Affiliations
Additional information
This research was supported in part by Otago Research Committee Grant 37885.
Rights and permissions
About this article
Cite this article
Gleeson, R.M., Dragunow, M.G., Kirton, N.F. et al. Intracranial cannulation of small animals. Behavior Research Methods & Instrumentation 12, 346–348 (1980). https://doi.org/10.3758/BF03201671
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.3758/BF03201671