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Haplotype TGTG from SNP 45T/G and 276G/T of the adiponectin gene contributes to risk of polycystic ovary syndrome

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Abstract

Background: Haplotypes of adiponectin gene single nucleotide polymorphisms (SNP) might be related to metabolic disorders. Aim: To assess whether the prevalence of SNP 45T/G and 276G/T of the adiponectin gene and their haplotypes differ between polycystic ovary syndrome (PCOS) and non-hirsute cycling controls and to investigate the relationship between these haplotypes and risk factors for cardiovascular disease. Subjects and methods: In this case-control study, 80 women with PCOS and 1500 non-hirsute controls with regular cycles underwent clinical and laboratory measurements. Genotype distribution was analyzed by conventional PCR-restriction fragment length polymorphism. Results: Compared to controls, PCOS women had greater body mass index (BMI) (31.0±7.9 kg/m2 vs 23.4±4.6 kg/m2; p<0.001), waist circumference (92.2±18.8 cm vs 74.5±10.2 cm; p<0.001), and systolic and diastolic blood pressure (124.6±19.9 vs 111.5±13.0 mmHg and 79.2±12.5 vs 71.8±10.6 mmHg; p<0.025), as well as a worse lipid profile (p<0.007), even after adjustment for age and BMI. Genotype distribution was similar in PCOS and controls (45T/G: p=0.399; 276G/T: p=0.135). Six haplotypes were inferred and their frequencies differed significantly between the groups (p=0.001). The TGTG haplotype was more frequent in PCOS than controls (41.3 vs 18.9%). In PCOS, the GG genotype for SNP 276 (p=0.031) and the TGTG haplotype (p=0.023) were associated with higher systolic blood pressure vs other genotypes and haplotypes. Body composition, glucose, insulin, and lipid profile were similar across genotypes and haplotypes in both groups. Conclusions: Haplotype TGTG from adiponectin gene variants 45T/G and 276G/T is related to susceptibility to PCOS, and might be associated with increased blood pressure in PCOS.

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Authors and Affiliations

  1. Gynecological Endocrinology Unit, Division of Endocrinology, Hospital de Clínicas de Porto Alegre (HCPA), Rua Ramiro Barcelos, 2350, 90035-003, Porto Alegre, RS, Brazil

    S. Radavelli-Bagatini, R. B. Ramos, B. R. Santos, S. B. Lecke & P. M. Spritzer MD, PhD

  2. Laboratory of Molecular Endocrinology, Department of Physiology, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    S. Radavelli-Bagatini, R. B. Ramos, B. R. Santos, S. B. Lecke & P. M. Spritzer MD, PhD

  3. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), National Institute of Hormones and Women’s Health, Porto Alegre, Brazil

    S. Radavelli-Bagatini, I. O. de Oliveira, R. B. Ramos, B. R. Santos, S. B. Lecke & P. M. Spritzer MD, PhD

  4. Department of Physiology and Pharmacology, Universidade Federal de Pelotas (UFPEL), Pelotas, Brazil

    I. O. de Oliveira & M. S. Wagner

  5. Post-Graduate Program in Epidemiology, Universidade Federal de Pelotas (UFPEL), Pelotas, Brazil

    D. P. Gigante & B. L. Horta

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  1. S. Radavelli-Bagatini
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  2. I. O. de Oliveira
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  9. P. M. Spritzer MD, PhD
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Correspondence to P. M. Spritzer MD, PhD.

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Radavelli-Bagatini, S., de Oliveira, I.O., Ramos, R.B. et al. Haplotype TGTG from SNP 45T/G and 276G/T of the adiponectin gene contributes to risk of polycystic ovary syndrome. J Endocrinol Invest 36, 497–502 (2013). https://doi.org/10.3275/8966

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