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Differential effects of down-regulated steroidogenic factor-1 on basal and angiotensin II-induced aldosterone secretion

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Abstract

Aldosterone synthase (CYP11B2) is responsible for the final step in aldosterone synthesis and is importantly regulated by angiotensin-II (Ang II) through diverse pathways. However, under pathological conditions, such as in hyperaldosteronism, the regulation becomes disordered. The transcription factor steroidogenic factor-1 (SF-1) is important in regulating the endocrine system and is overexpressed in aldosterone-producing adenoma (APA), a common cause of hyperaldosteronism. Overexpression of SF-1 has been extensively studied, but little in-depth information is available regarding the effects of inhibitory SF-1 on CYP11B2 and Ang II. In this paper, we have investigated the roles of down-regulated SF-1 in basal and Ang II-induced CYP11B2 expression using SF-1-specific short hairpin RNA. Inhibitory SF-1 was found to decrease the sensitivity of CYP11B2 and aldosterone to Ang II stimulation, whereas a down-regulation of SF-1 enhanced basal CYP11B2 expression and aldosterone production in H295R cells. Considering these differential effects of SF-1 on aldosterone production, these results might provide a new insight into the understanding of hyperaldosteronism.

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Author notes

  1. D. Hu

    Present address: Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China

Authors and Affiliations

  1. Department of Endocrinology, Chinese PLA General Hospital, Beijing, China

    J. Ouyang

  2. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    D. Hu & S. Wang

  3. Department of Urology, General Hospital of People’s Liberation Army, 28 Fuxin Road, 100853, Beijing, China

    B. Wang, T. Shi, X. Ma, H. Li & X. Zhang MD

Authors
  1. J. Ouyang
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  2. D. Hu
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  7. S. Wang
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  8. X. Zhang MD
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Corresponding author

Correspondence to X. Zhang MD.

Additional information

J.O., D.H., B.W. contributed equally to this work.

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Ouyang, J., Hu, D., Wang, B. et al. Differential effects of down-regulated steroidogenic factor-1 on basal and angiotensin II-induced aldosterone secretion. J Endocrinol Invest 34, 671–675 (2011). https://doi.org/10.3275/7413

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