Skip to main content

Advertisement

SpringerLink
Log in

Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes

  • Original Articles
  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

Background: The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes. Aim: The aim of this study was to investigate the effect of 17β-estradiol on transepithelial electrical resistance in the human amniochorion. Material and methods: Specimens of human fetal membranes were obtained. 17β-estradiol, tamoxifen and their combination were added in an Ussing chamber. Transepithelial resistance was measured before and after the addition of each solution. Results: An increase in transepithelial resistance was seen after the addition of estradiol to both sides of the membranes. The effect was rapid with a peak at the 1st min of application and dose-depended. Tamoxifen, caused a similar effect but smaller in magnitude and shorter in duration. Tamoxifen in combination with estradiol inhibited only in part the action of estradiol. Conclusions: These results suggest that estradiol induces a rapid increase of transepithelial resistance in human fetal membranes in vitro via a non-genomic pathway. It is possible those changes in transepithelial resistance play a role in the control of permeability of human amniochorion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mitchell BF, Cross J, Hobkirk R, Challis JR. Formation of unconjugated estrogens from estrone sulfate by dispersed cells from human fetal membranes and decidua. J Clin Endocrinol Metab 1984, 58: 845–9.

    Article  PubMed  Google Scholar 

  2. Petraglia F, Florio P, Nappi C, Genazzani AR. Peptide signaling in human placenta and membranes: autocrine, paracrine, and endocrine mechanisms. Endocr Rev 1996, 17: 156–86.

    PubMed  Google Scholar 

  3. Fiorini S, Ferretti ME, Biondi C, et al. 17Beta-eEstradiol stimulates arachidonate release from human amnion-like WISH cells through a rapid mechanism involving a membrane receptor. Endocrinology 2003, 144: 3359–67.

    Article  PubMed  Google Scholar 

  4. Bukovsky A, Caudle MR, Cekanova M, et al. Placental expression of estrogen receptor beta and its hormone binding variant—comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells. Reprod Biol Endocrinol 2003, 1: 36.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Wintour EM, Laurence BM, Lingwood BE. Anatomy, physiology and pathology of the amniotic and allantoic compartments in the sheep and cow. Aust Vet J 1986, 63: 216–21.

    Article  PubMed  Google Scholar 

  6. Rice GE, Hoier R, Christensen P, Dantzer V, Skadhauge E. Endocrine correlates of placental permeability during gestation in the pig. Comp Biochem Physiol Comp Physiol 1993, 105: 111–7.

    Article  PubMed  Google Scholar 

  7. Harvey BJ, Alzamora R, Healy V, Renard C, Doolan CM. Rapid responses to steroid hormones: from frog skin to human colon. A homage to Hans Ussing. Biochim Biophys Acta 2002, 1566: 116–28.

    Article  PubMed  Google Scholar 

  8. Gorodeski GI, Goldfarb J. Seminal fluid factor increases the resistance of the tight junctional complex of cultured human cervical epithelium CaSki cells. Fertil Steril 1998, 69: 309–17.

    Article  PubMed  Google Scholar 

  9. Verikouki CH, Hatzoglou CH, Gourgoulianis KI, Molyvdas PA, Kallitsaris A, Messinis IE. Rapid effect of progesterone on transepithelial resistance of human fetal membranes: evidence for non-genomic action. Clin Exp Pharmacol Physiol 2008, 35: 174–9.

    PubMed  Google Scholar 

  10. Guiet-Bara A, Bara M. Comparative study of the human amnion, chorion and chorioamnion permeability to monovalent cations. Eur J Obstet Gynecol Reprod Biol 1983, 16: 1–7.

    Article  PubMed  Google Scholar 

  11. Hardy MA, Leonardi RT, Scheide JI. Cellular permeation pathways in a leaky epithelium: the human amniochorion. Biol Cell 1989, 66: 149–53.

    Article  PubMed  Google Scholar 

  12. Hatzoglou C, Gourgoulianis KI, Hatzoglou A, Castanas E, Molyvdas PA. Rapid effects of 17beta-estradiol and progesterone on sheep visceral and parietal pleurae via a nitric oxide pathway. J Appl Physiol 2002, 93: 752–8.

    PubMed  Google Scholar 

  13. Pietras RJ, Szego CM. Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells. Nature 1977, 265: 69–72.

    Article  PubMed  Google Scholar 

  14. Dufy B, Vincent JD, Fleury H, Du Pasquier P, Gourdji D, Tixier-Vidal A. Membrane effects of thyrotropin-releasing hormone and estrogen shown by intracellular recording from pituitary cells. Science 1979, 204: 509–11.

    Article  PubMed  Google Scholar 

  15. Tesarik J, Mendoza C. Nongenomic effects of 17 beta-estradiol on maturing human oocytes: relationship to oocyte developmental potential. J Clin Endocrinol Metab 1995, 80: 1438–43.

    PubMed  Google Scholar 

  16. Sömjen D, Kohen F, Lieberherr M. Nongenomic effects of an anti-idiotypic antibody as an estrogen mimetic in female human and rat osteoblasts. J Cell Biochem 1997, 65: 53–66.

    Article  PubMed  Google Scholar 

  17. McNamara B, Winter DC, Cuffe J, Taylor C, O’Sullivan GC, Harvey BJ. Rapid activation of basolateral potassium transport in human colon by oestradiol. Br J Pharmacol 2000, 131: 1373–8.

    Article  PubMed Central  PubMed  Google Scholar 

  18. Schmidt BM, Gerdes D, Feuring M, Falkenstein E, Christ M, Wehling M. Rapid, nongenomic steroid actions: A new age? Front Neuroendocrinol 2000, 21: 57–94.

    Article  PubMed  Google Scholar 

  19. Falkenstein E, Norman AW, Wehling M. Mannheim classification of nongenomically initiated (rapid) steroid action(s). J Clin Endocrinol Metab 2000, 85: 2072–5.

    Article  PubMed  Google Scholar 

  20. Chen Y, Schindler M, Simon SM. A mechanism for tamoxifen-mediated inhibition of acidification. J Biol Chem 1999, 274: 18364–73.

    Article  PubMed  Google Scholar 

  21. Lehenkari P, Parikka V, Rautiala TJ, et al. The effects of tamoxifen and toremifene on bone cells involve changes in plasma membrane ion conductance. J Bone Miner Res 2003, 18: 473–81.

    Article  PubMed  Google Scholar 

  22. Kallio A, Zheng A, Dahllund J, Heiskanen KM, Härkönen P. Role of mitochondria in tamoxifen-induced rapid death of MCF-7 breast cancer cells. Apoptosis 2005, 10: 1395–410.

    Article  PubMed  Google Scholar 

  23. Porta M, Capurro C, Parisi M. Water permeability in the human amnion: pH regulation of the paracellular pathway. Biochim Biophys Acta 1989, 980: 220–4.

    Article  PubMed  Google Scholar 

  24. Cruz Silva MM, Madeira VM, Almeida LM, Custodio JB. Hydroxy-tamoxifen interaction with human erythrocyte membrane and induction of permeabilization and subsequent hemolysis. Toxicol In Vitro 2001, 15: 615–22.

    Article  PubMed  Google Scholar 

  25. Cano A, Hermenegildo C. Modulation of the oestrogen receptor: a process with distinct susceptible steps. Hum Reprod Update 2000, 6: 207–11.

    Article  PubMed  Google Scholar 

  26. Harvey BJ, Condliffe S, Doolan CM. Sex and salt hormones: rapid effects in epithelia. News Physiol Sci 2001, 16: 174–7.

    PubMed  Google Scholar 

  27. Moreno AS, Franci CR. Estrogen modulates the action of nitric oxide in the medial preoptic area on luteinizing hormone and prolactin secretion. Life Sci 2004, 74: 2049–59.

    Article  PubMed  Google Scholar 

  28. Lemancewicz A, Laudanska H, Laudanski T, Karpiuk A, Batra S. Permeability of fetal membranes to calcium and magnesium: possible role in preterm labour. Hum Reprod 2000, 15: 2018–22.

    Article  PubMed  Google Scholar 

  29. Gorodeski GI. Estrogen biphasic regulation of paracellular permeability of cultured human vaginal-cervical epithelia. J Clin Endocrinol Metab 2001, 86: 4233–43.

    Article  PubMed  Google Scholar 

  30. Ye L, Martin TA, Parr C, Harrison GM, Mansel RE, Jiang WG. Biphasic effects of 17-beta-estradiol on expression of occludin and transendothelial resistance and paracellular permeability in human vascular endothelial cells. J Cell Physiol 2003, 196: 362–9.

    Article  PubMed  Google Scholar 

  31. Gorodeski GI. Aging and estrogen effects on transcervical-transvaginal epithelial permeability. J Clin Endocrinol Metab 2005, 90: 345–51.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Thessalia, 22 Papakiriazi Street, 41222, Larissa, Greece

    C. Verikouki, A. I. Zavos, A. Kallitsaris & I. E. Messinis MD, PhD

  2. Department of Physiology, Medical School, University of Thessalia, Larissa, Greece

    C. Hatzoglou, K. I. Gourgoulianis & P. A. Molyvdas

Authors
  1. C. Verikouki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Hatzoglou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. I. Zavos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. I. Gourgoulianis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. A. Molyvdas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Kallitsaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. E. Messinis MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. E. Messinis MD, PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verikouki, C., Hatzoglou, C., Zavos, A.I. et al. Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes. J Endocrinol Invest 34, 510–514 (2011). https://doi.org/10.3275/7206

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3275/7206

Key-words

Search

Navigation