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Identification of herpes simplex virus type 1 proteins encoded within the first 1.5 kb of the latency-associated transcript

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Abstract

Expression of the first 1.5 kb of the latency-associated transcript (LAT) that is encoded by herpes simplex virus type 1 (HSV-1) is sufficient for wild-type (wt) levels of reactivation from latency in small animal models. Peptide-specific immunoglobulin G (IgG) was generated against open reading frames (ORFs) that are located within the first 1.5 kb of LAT coding sequences. Cells stably transfected with LAT or trigeminal ganglionic neurons of mice infected with a LAT expressing virus appeared to express the L2 or L8 ORF. Only L2 ORF expression was readily detected in trigeminal ganglionic neurons of latently infected mice.

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Authors and Affiliations

  1. Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska, 68503, Lincoln, NE, USA

    Gail Henderson & Clinton Jones

  2. School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, USA

    Tareq Jaber & Clinton Jones

  3. The Gavin Herbert Eye Institute, School of Medicine, University of California Irvine, Irvine, California, USA

    Dale Carpenter & Steven L. Wechsler

  4. Department of Microbiology and Molecular Genetics, School of Medicine, and Center for Virus Research, University of California Irvine, Irvine, California, USA

    Steven L. Wechsler

Authors
  1. Gail Henderson
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  2. Tareq Jaber
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  3. Dale Carpenter
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  4. Steven L. Wechsler
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  5. Clinton Jones
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Corresponding author

Correspondence to Clinton Jones.

Additional information

Dr. C. Jones’ laboratory is supported by a PHS grant (R21AI069176), two USDA grants (2009- and 2006-01627), and a PHS grant 1P20RR15635 to the Nebraska Center for Virology. Dr. Wechsler’s laboratory is supported by PHS grant EY13191, The Discovery Eye Foundation, The Henry L. Guenther Foundation, and Research to Prevent Blindness. Dr. Wechsler is a RPB Senior Scientific Investigator.

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Henderson, G., Jaber, T., Carpenter, D. et al. Identification of herpes simplex virus type 1 proteins encoded within the first 1.5 kb of the latency-associated transcript. Journal of NeuroVirology 15, 439–448 (2009). https://doi.org/10.3109/13550280903296353

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  • DOI: https://doi.org/10.3109/13550280903296353

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