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Possibilities of the Spectrometry Method in Determining the Sex of Hatching Egg Embryos

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Abstract

The culling of day-old male chicks from laying hens remains a common procedure during incubation in hatchers in Russian poultry farms. It is not only unethical but also causes economic damage to poultry producers. A number of European countries have already introduced laws prohibiting the killing of one-day-old male chicks and are developing measures to limit the killing of live embryos in poultry eggs after six days of incubation. Producers are now obliged to use technology to determine the sex of a chick before it is born. From 2024, there will be a ban on killing live embryos in a poultry egg after the 6th day of incubation. The determination of the sex of an embryo in the egg before incubation remains an unresolved problem. In this regard, intensive research is being carried out to find the relationship between the morphological parameters of a freshly laid poultry egg and the sexual dimorphism of its embryo. An approach based on analysis of hyperspectral images of eggs by radiation transillumination in a wide range of waves is proposed. A setup based on a Photonfocus MV1-D2048x1088-HS05-96-G2-10 hyperspectral camera with an IMEC CMV2K-LS150-VNIR sensor enabling local illumination of a study object with an adjustable radiation source has been created and debugged for implementing this approach. Morphological parameters such as weight, area, volume, and shape ratio of eggs are not correlated with the sex of the embryo of an egg before incubation. Informative wavelengths for further studies to determine the sexual dimorphism of chicken egg embryos in the first days of incubation are 640, 660, and 688 nm.

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REFERENCES

  1. Burke, W.H. and Sharp, P.J., Sex differences in body weight of chicken embryos, Poult. Sci., 1989, vol. 68, pp. 805–810. https://doi.org/10.3382/ps.0680805

    Article  CAS  PubMed  Google Scholar 

  2. Burke, W.H., Sex differences in incubation length and hatching weights of broiler chick, Poult. Sci., 1992, vol. 71, pp. 1933–1938. https://doi.org/10.3382/ps.0711933

    Article  CAS  PubMed  Google Scholar 

  3. Galli, R., Preusse, G., Schnabel, C., et al., Sexing of chicken eggs by fluorescence and Raman spectroscopy through the shell membrane, PLoS One, 2018, vol.  139, no. 2, p. e0192554. https://journals.plos.org/plosone/article?id=https://doi.org/10.1371/journal.pone.0192554. 10.1371/journal.pone.0192554

  4. Khaliduzzaman, A., Fujitani, S., Kashimori, A., et al., Non-invasive broiler chick embryo sexing based on opacity value of incubated eggs, Comp. Electron. Agricult., 2019, vol. 158, pp. 30–35. https://doi.org/10.1016/j.compag.2019.01.029

    Article  Google Scholar 

  5. Infosperber SSUI, 2022, Gschweng, D., In Deutschland ist es seit 2022 verboten, Kuken zu schreddern. Die Industrie umgeht das Verbot, 2023. https://www.infosperber.ch/gesundheit/ernaehrung/bruderhaehne-toeten-verboten-nun-sterben-sie-im-ausland/. Cited February 10, 2023.

  6. Yannakopoulos, A.L. and Tserveni-Gousi, A.S., Quality characteristics of quail eggs, Br. Poult. Sci., 1986, vol. 27, no. 2, pp. 171–176. https://doi.org/10.1080/00071668608416870

    Article  Google Scholar 

  7. Vasilenko, N.V., Ivashov, E.N., Protsenko, V.V., et al., RF Patent 2238643, 2004.

  8. Steiner, G., Bartels, T., Stelling, A., et al., Gender determination of fertilized unincubated chicken eggs by infrared spectroscopic imaging, Anal. Bioanal. Chem., 2011, vol. 400, no. 9, pp. 2775–2782. https://doi.org/10.1007/s00216-011-4941-3

    Article  CAS  PubMed  Google Scholar 

  9. Weissmann, A., Reitemeier, S., Hahn, A., et al., Sexing domestic chicken before hatch: A new method for in ovo gender identification, Theriogenology, 2013, vol. 80, no. 3, pp. 199–205. https://doi.org/10.1016/j.theriogenology.2013.04.014

    Article  CAS  PubMed  Google Scholar 

  10. Gohler, D., Fischer, B., and Meissner, S., In-ovo sexing of 14-day-old chicken embryos by pattern analysis in hyperspectral images (VIS/NIR spectra): A non-destructive method for layer lines with gender-specific down feather color, Poult. Sci., 2017, vol. 96, no. 1, pp. 1–4. https://www.sciencedirect.com/science/article/pii/S0032579119312398?via%3Dihub. https://doi.org/10.3382/ps/pew282

    Article  CAS  PubMed  Google Scholar 

  11. Okinda, C., Lu, M., Liu, L., et al., A review on computer vision systems in monitoring of poultry: A welfare perspective, Artif. Intell. Agric., 2020. vol. 4. pp. 184–208. https://doi.org/10.1016/j.aiia.2020.09.002

    Article  Google Scholar 

  12. Fujiyoshi, H., Hirakawa, T., and Yamashita, T., Deep learning-based image recognition for auton-omous driving, Int. Assoc. Traffic Saf. Sci. Res., 2019, vol. 43, pp. 244–252. https://doi.org/10.1016/j.iatssr.2019.11.008

    Article  Google Scholar 

  13. Zhu, Z.H., Ye, Z.F., and Tan, Y., Non-destructive identification for gender of chicken eggs based on GA-BPNN with double hidden layers, J. Appl. Poult. Res., 2021, vol. 30, no. 4, p. 100203. https://www.sciencedirect.com/science/article/pii/S1056617121000660?via%3Dihub. https://doi.org/10.1016/j.japr.100203

  14. Feng, Z., Ding, C.Q., Li, W.H., et al., Detection of blood spots in eggs by hyperspectral transmittance imaging, Int. J. Agric. Biol. Eng., 2019, vol. 12, no. 6, pp. 209–214. https://doi.org/10.25165/j.ijabe.20191205.5376

    Article  Google Scholar 

  15. Corion, M., Keresztes, J., Ketelaere, B., et al., In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration, Poult. Sci., 2022, vol. 101, p. 101782. https://www.sciencedirect.com/science/article/pii/S0032579122000906?via%3Dihub. https://doi.org/10.1016/j.psj.2022.101782

  16. Klimenko, D.N., Gurova, T.A., Elkin, O.V., et al., Hyperspectral reflectance system for plant diagnostics, IOP Conf. Ser.: Earth Environ. Sci., 2020, vol. 48, no. 3, p. 032019. https://iopscience.iop.org/article/https://doi.org/10.1088/1755-1315/548/3/032019. 10.1088/1755-1315/548/3/032019

  17. Aleinikov, A.F., Hyperspectral equipment for determining the sexual dimorphism of the chick embryo during incubation, in Pishcha. Ekologiya. Kachestvo (Food. Ecology. Quality), Krasnoobsk: Sib. Fed. Nauchn. Tsentr Agrobiotekhnol. Ross. Akad. Nauk, 2022, pp. 42–46.

  18. Aleinikov, A.F., Methods for non-invasive evaluation of sexual dimorphism of embryos in bird eggs, Sib. Vestn. S-kh. Nauki, 2022, vol. 52, no. 5, pp. 105–116. https://doi.org/10.26898/0370-8799-2022-5-13

    Article  Google Scholar 

  19. Hoyt, D.F., Practical methods of estimating volume and fresh weight of bird eggs, Auk, 1979, vol. 96, no. 1, pp. 73–77.

    Google Scholar 

  20. Yilmaz-Dikmen, B. and Dikmen, S., A morphometric method of sexing white layer eggs, Braz. J. Poult. Sci., 2013, vol. 15, pp. 203–210. https://doi.org/10.1590/S1516-635X2013000300006

    Article  Google Scholar 

  21. Yannakopoulos, A.L. and Tserveni-Gousi, A.S., Quality characteristics of quail eggs, Br. Poult. Sci., 1986, vol. 27, no. 2, pp. 171–176. https://doi.org/10.1080/00071668608416870

    Article  Google Scholar 

  22. Wang, X., Xu, L., Chen, H., et al., Non-destructive detection of pH value of kiwifruit based on hyperspectral fluorescence imaging technology, Agriculture, 2022, vol. 12, no. 2, p. 208. https://www.mdpi.com/2077-0472/12/2/208. https://doi.org/10.3390/agriculture12020208

    Article  CAS  Google Scholar 

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Funding

This study was supported by the Russian Science Foundation, grant no. 22-26-00198.

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Authors and Affiliations

  1. Siberian Federal Research Center of Agricultural Biotechnology, Russian Academy of Sciences, 630501, Krasnoobsk, Russia

    A. F. Aleinikov & A. F. Cheshkova

  2. Novosibirsk State Technical University, 630092, Novosibirsk, Russia

    A. F. Aleinikov & I. V. Osipenko

  3. Technological Design Institute of Scientific Instrument Engineering, Siberian Branch, Russian Academy of Sciences, 630058, Novosibirsk, Russia

    E. S. Smirnov

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  1. A. F. Aleinikov
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  2. I. V. Osipenko
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  3. A. F. Cheshkova
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  4. E. S. Smirnov
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Correspondence to A. F. Aleinikov.

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Translated by D. Zabolotny

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Aleinikov, A.F., Osipenko, I.V., Cheshkova, A.F. et al. Possibilities of the Spectrometry Method in Determining the Sex of Hatching Egg Embryos. Russ. Agricult. Sci. 49 (Suppl 2), S356–S362 (2023). https://doi.org/10.3103/S1068367423080025

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  • DOI: https://doi.org/10.3103/S1068367423080025

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