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Tomato Seed Borne Ralstonia solanacearum in Bangladesh

  • PLANT GROWING, PLANT PROTECTION AND BIOTECHNOLOGY
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Abstract

Genetic diversity of Ralstonia solanacearum, on seeds of tomato from different seed sources in Bangladesh was studied by using Random amplified polymorphic DNA (RAPD) markers. The primers viz., OPA02, OPA08, OPA09, OPA10, OPA18 and OPE19 were tested in this experiment and were also evaluated on the basis of intensity and resolution of the band. Of these, the specific marker (OPA18) of RAPD in molecular characterisation of R. solanacearum produced distinct twenty bands with 100% polymorphism at base pair of 100–2000. Segregation of isolates of R. solanacearum was determined in unweighted pair group method with arithmetic means. In cluster analysis, isolate of Metal seed company Ltd. was very near with United seed company limited and Mallika seed company with the least genetic distance. Metal seed company Ltd. was also close to Lal Teer seed company Ltd. with lower genetic distance (0.1625). Coefficient of gene differentiation (1.0000) in R. solanacearum marked high genetic variations indicating adequate polymorphism. The highest (1.204) genetic distance was found in Namdhari seed company limited vs. Bangladesh Institute of Nuclear Agriculture (BINA). Moreover genetic identity was obtained as higher (0.8500) in United seed company limited vs. Metal seed company Ltd., and the lowest genetic identity (0.3000) was noted in BINA vs. Namdhari seed company limited. Banding pattern, gene differentiation, genetic distance and genetic identity showed the distinct genetic variations which were associated with each isolate of R. solanacearum.

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ACKNOWLEDGMENTS

The authors acknowledge the financial support and scholarship granted by the Bangabandhu fellowship on Science, Information and Communication Technology, Ministry of Science and Technology, Dhaka and also acknowledge the Professor Golam Ali Fakir Seed Pathology Centre and Molecular Plant Pathology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh to provide laboratory facilities.

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Authors and Affiliations

  1. Golam Ali Fakir Seed Pathology Centre, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh

    Purnima Dey

  2. College of Agricultural sciences, International University of Business Agriculture and Technology (IUBAT), Uttara Model Town, 1230, Dhaka, Bangladesh

    Ismail Hossain

  3. Department of Agricultural Extension, Kushtia, Bangladesh

    Hyat Mahmud

  4. World Vision Bangladesh, Dhaka, Bangladesh

    Sajal Kumer Dey

  5. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

    Mohammad Delwar Hossain

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  1. Purnima Dey
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Correspondence to Hyat Mahmud.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Purnima Dey, Hossain, I., Mahmud, H. et al. Tomato Seed Borne Ralstonia solanacearum in Bangladesh. Russ. Agricult. Sci. 47, 386–393 (2021). https://doi.org/10.3103/S1068367421040157

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  • DOI: https://doi.org/10.3103/S1068367421040157

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