Development of reliable fertility-restoring lines is the most important prerequisite for using cytoplasmic male sterility (CMS) in plant breeding. Sorghum 9E type CMS is characterized by unstable expression of fertility-restoring genes, which require high levels of water availability for plants during microsporogenesis and flowering. In order to create reliable male-fertility-restoring lines for 9E type CMS, the progeny of test crosses of fertile revertant lines in 9E cytoplasm, which were obtained as a result of reversions in sterile F1 hybrids induced by conditions of high water availability, with CMS lines in this cytoplasm were grown in selective backgrounds: in the dry plots and in the plots with additional watering as well as in the field under natural moisture supply. In the majority of hybrid combinations, fertile plants were observed only when additionally watered during the flowering period. F1 hybrids with different CMS lines with 9E cytoplasm significantly differed in terms of the degree of restored fertility and the response to water availability conditions. Fertile plants predominated in the F2 families; in different hybrid combinations, the ratio of restored and sterile plants corresponded to 3 : 1 or 13 : 3 segregation. After selection in the drought conditions of the fertile plants from the offspring of hybrids from the cross of the CMS line 9E Pishchevoe 614 and male-fertile revertants, two lines were obtained that were able to restore fertility of F1 hybrids in field conditions.
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Chen, L. and Liu, Y.-G., Male sterility and fertility restoration in crops, Annu. Rev. Plant Biol., 2014, vol. 65, pp. 579–606.
Bohra, A., Jha, U.C., Adhimoolam, P., Bisht, D., and Singh, N.P., Cytoplasmic male sterility (CMS) in hybrid breeding in field crops, Plant Cell Rep., 2016, vol. 35, pp. 967–993. https://doi.org/10.1007/s00299-016-1949-3
Jacoby, R.P., Li, L., Huang, S., Millar, A.H., and Taylor, N.L., Mitochondrial composition, function and stress response in plants, J. Integr. Plant Biol., 2012, vol. 54, pp. 887–906. https://doi.org/10.1111/j.1744-7909.2012.01177.x
Liberator, K.L., Dukowic-Schulze, S., Miller, M.E., Chen, C., and Kianian, S.F., The role of mitochondria in plant development and stress tolerance, Free Radical Biol. Med., 2016, vol. 100, pp. 238–256.
Tracy, W.F., Everett, H.L., and Gracen, V.E., Inheritance, environmental effects and partial male fertility in C-type CMS in a maize inbred, J. Hered., 1991, vol. 82, pp. 343–346.
Gabay-Laughnan, S., Kuzmin, E.V., Monroe, J., Roark, L., and Newton, K.J., Characterization of a novel thermo-sensitive restorer of fertility for cytoplasmic male sterility in maize, Genetics, 2009, vol. 182, pp. 91–103.
Weider, C., Stamp, P., Christov, N., Husken, A., Foueillassar, X., Camp, K.-H., and Munsch, M., Stability of cytoplasmic male sterility in maize under different environmental conditions, Crop Sci., 2009, vol. 49, pp. 77–84.
Bückmann, H., Thiele, K., and Schiemann, J., Influence of soil moisture and air temperature on the stability of cytoplasmic male sterility (CMS) in maize (Zea mays L.), Agric. Sci., 2016, vol. 7, pp. 70–81. https://doi.org/10.4236/as.2016.72007
Sarvella, P., Environmental influences on sterility in cytoplasmic male-sterile cottons, Crop Sci., 1966, vol. 6, pp. 361–364.
Marshall, D.R., Thomson, N.J., Nicholls, G.H., and Patrick, C.M., Effects of temperature and day-length on cytoplasmic male sterility in cotton (Gossypium), Aust. J. Agric. Res., 1974, vol. 25, pp. 443–447.
Fan, Z.G. and Stefansson, B.R., Influence of temperature on sterility of 2 cytoplasmic male-sterility systems in rape (Brassica napus L.), Can. J. Plant Sci., 1986, vol. 66, pp. 221–227.
Reddy, B.V.S., Ramesh, S., and Ortiz, R., Genetic and cytoplasmic-nuclear male sterility in sorghum, Plant Breed. Rev., 2005, vol. 25, pp. 139–169.
Elkonin, L.A., Kozhemyakin, V.V., and Ishin, A.G., Influence of water availability on fertility restoration of CMS lines with the 'M35', A4 and '9E' CMS-inducing cytoplasms of sorghum, Plant Breed., 2005, vol. 134, pp. 565–571.
Elkonin, L.A. and Tsvetova, M.I., Heritable effect of plant water availability conditions on restoration of male fertility in the “9E” CMS-inducing cytoplasm of sorghum, Front. Plant Sci., 2012, vol. 3, p. 91. https://doi.org/10.3389/fpls.2012.00091
Elkonin, L.A., Gerashchenkov, G.A., Domanina, I.V., and Rozhnova, N.A., Inheritance of reversions to male fertility in male-sterile sorghum hybrids in the '9E' cytoplasm induced by environmental conditions, Russ. J. Genet., 2015, vol. 51, pp. 251–261.
Lakin, G.F., Biometriya (Biometrics), Moscow: Vyssh. Shk., 1990.
The authors declare that they have no conflict of interests. This article does not contain any studies involving animals or human participants performed by any of the authors.
Translated by K. Lazarev
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Elkonin, L.A., Sarsenova, S.K. Development of Fertility-Restoring Lines for 9E Type CMS of Sorghum Using Environmentally Induced Fertility Revertants. Russ. Agricult. Sci. 46, 560–565 (2020). https://doi.org/10.3103/S1068367420060063
- cytoplasmic male sterility
- fertility-restoring genes