Changes in Parameters of Fresh and Deconserved Sperm of Stallions after Their Vaccination against Anthrax

Abstract

The aim of this work was to estimate the effect of vaccinating stallions against anthrax on the sperm quality and its criostability. Vaccination of stallions against anthrax led to a decrease in the sperm quality. During the first week after vaccination, the sperm productivity decreased by 10%. In freshly diluted sperm, the sperm motility decreased by 12%, survival by 38% (Р > 0.99), and membrane integrity by 20% (Р > 0.95) on average. Their recovery was observed not earlier than 28 days after vaccination. Vaccination had an especially strong effect on sperm quality after cryopreservation: sperm motility decreased by 35% (P > 0.99), survival by 45% (P > 0.99), and membrane integrity by 46% (P > 0.99) on average, and these parameters remained low for 1 month. Rejection of sperm doses in the postvaccination period was 50% because of the decrease in quality below the standard level. The amount of pathological types of sperm increased 45 days after vaccination: by 27% (Р > 0.95) in fresh sperm and 34% (Р > 0.99) in deconserved sperm. In the case of cryoconservation of stallions' sperm, it is necessary to take into account the recovery period after antiepizootic measures.

This is a preview of subscription content, access via your institution.

Fig. 1.
Fig. 2.

REFERENCES

  1. 1

    Skatkin, P.N., Increasing the fertility of stallions, Konevodstvo, 1951, no. 6, pp. 9–15.

  2. 2

    Naumenkova, V.A., The optimal age of stallions for effective cryopreservation of sperm, Konevod. Konnyi Sport, 2016, no. 6, p. 13.

  3. 3

    Atroshchenko, M.M. and Bragina, E.E., The effect of cryopreservation on the ultrastructure of spermatozoa in stallions of different age groups, in Nauchnoe obespechenie innovatsii razvitiya zhivotnovodstva (Scientific Support for Livestock Development Innovations), Zhodino, 2013, pp. 11–12.

  4. 4

    Naumenkova, V.A., Kalashnikov, V.V., Zaitsev, A.V., Atroshchenko, M.M., and Kalashnikova, T.V., Dynamics of qualitative indicators of semen in stallions of adjacent generations, Konevod. Konnyi Sport, 2018, no. 2, pp. 32–35.

  5. 5

    Andryushin, V.V., Fomina, E.L., and Miroshnikova, K.I., Spermatogenesis and resistance of sperm to freezing in association to the level of steroid hormones, in Puti uskoreniya nauchno-tekhnicheskogo progressa v konevodstve (Ways to Accelerate Scientific and Technological Progress in Horse Breeding), 1986, pp. 112–120.

    Google Scholar 

  6. 6

    Nikitkina, E.V., Shapiev, I.Sh., Platonova, N.P., and Naumenkova, V.A., Variability of stallion sperm quality indicators, Aktual’nye problemy biologii vosproizvodstva zhivotnykh: Materialy mezhdunarodnoi nauchno-prakticheskoi konferentsii (Current Problems of Animal Reproduction Biology: Proc. Int. Sci.-Pract. Conf.), Dubrovitsy-Bykovo, 2007, pp. 476–478.

  7. 7

    Fedosova, O.A., Terekhina, A.A., and Bakovetskaya, O.V., Monitoring the dynamics of sex hormones in horses by the seasons of the year, Konevod. Konnyi Sport, 2010, no. 2, pp. 29–30.

  8. 8

    Fomichev, Yu.P., The value and assessment of environmental factors in the biology of reproduction of farm animals, Aktual’nye problemy biologii vosproizvodstva zhivotnykh: Materialy mezhdunarodnoi nauchno-prakticheskoi konferentsii (Current Problems of Animal Reproduction Biology: Proc. Int. Sci.-Pract. Conf.), Dubrovitsy-Bykovo, 2007, pp. 90–102.

  9. 9

    Lebedeva, L.F., Atroshchenko, M.M., and Burmistrova, S.A., The main factors affecting the effectiveness of insemination of mares with sperm cryopreserved according to Russian and foreign technologies, S-kh. Biol., 2015, no. 4, pp. 476–485.

  10. 10

    Kislenko, V.N., Kolychev, N.M., and Gosmanov, R.G, Veterinarnaya mikrobiologiya i immunologiya (Veterinary Microbiology and Immunology), GEOTAR-Media, 2012.

  11. 11

    Anokhina, N.V., Obshchaya i klinicheskaya immunologiya (General and Clinical Immunology), Moscow: Eksmo, 2008.

  12. 12

    Manuilov, A.V. and Nezhdanov, A.G., The problem of reducing the reproductive capacity of cattle during preventive vaccination against infectious diseases, Aktual’nye problemy biologii vosproizvodstva zhivotnykh: Materialy mezhdunarodnoi nauchno-prakticheskoi konferentsii (Current Problems of Animal Reproduction Biology: Proc. Int. Sci.-Pract. Conf.), Dubrovitsy-Bykovo, 2007, pp. 238–241.

  13. 13

    Moskvina, A.S., Maksimov, V.I., and Verkhovskikh, O.A., Morphophysiological indicators of blood of down-calving cows after vaccination, Vestn. Orlov. Gos. Agrarn. Univ., 2011, no. 6, pp. 65–67.

  14. 14

    Rekomendatsii po vzyatiyu, razbavleniyu i zamorazhivaniyu spermy zherebtsov (Recommendations for Collecting, Diluting, and Freezing Semen of Stallions). Divovo, 2006.

  15. 15

    Instruktsiya po iskusstvennomu osemeneniyu i transplantatsii embrionov (Instructions on Artificial Insemination and Transplantation of Embryos), Divovo, 2012.

  16. 16

    England, G.C. and Plummer, J.M., Hypo-osmotic swelling of dog spermatozoa, J. Reprod. Fertil., 1993, suppl. 47, pp. 261–270.

  17. 17

    Milovanov, V.K., Biologiya vosproizvedeniya i iskus stvennoe osemenenie zhivotnykh (Reproduction Biology and Artificial Insemination of Animals), Moscow: Sel’khozizdat, 1962.

  18. 18

    Pakenas, P.I., The study of spermatogenesis and improvement of the technology for collecting and processing the semen, Extended Abstract of Doctoral (Biol.) Dissertation, Tallinn, 1972.

  19. 19

    McKinnon, A.O., Squires, E.L., Vaala, W.E., and Varner, D.D., Equine Reproduction, John Wiley-Dlackwell, 2011, 2nd ed.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to V. A. Naumenkova.

Ethics declarations

Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Translated by K. Lazarev

About this article

Verify currency and authenticity via CrossMark

Cite this article

Naumenkova, V.A., Kalinova, A.V. Changes in Parameters of Fresh and Deconserved Sperm of Stallions after Their Vaccination against Anthrax. Russ. Agricult. Sci. 45, 589–592 (2019). https://doi.org/10.3103/S1068367419060168

Download citation

Keywords:

  • stallions
  • sperm
  • survivability
  • cryoconservation
  • vaccine
  • anthrax