Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench


A tissue culture method has been used for the breeding of buckwheat (Fagopyrum esculentum Moench) in the presence of high concentrations of heavy metal ions in a nutrient medium as selective factors. Based on the Izumrud (Russia) and Kitawase (Japan) varieties and their hybrid (Izumrud × Kitawase), some copper- and zinc-tolerant regenerant lines have been derived. The negative effect of copper ions in the form of chlorophyll mutants has been observed starting from the second generation of regenerant plants. A positive effect of heavy metals on the increase of a rutin content in regenerants has been observed under in vitro conditions. The field evaluation of heavy metal-tolerant samples has allowed us to select lines highly adapted to the monsoon climate of Primorsky krai and to combine such features as large grain size (thousand kernel weight is equal to 36–38 g), high seed productivity (1.9–3.2 g), and high rutin content in green mass (2.8–4.3%).

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  1. 1

    A Comprehensive Program for the Development of Biotechnology in the Russian Federation for the Period until 2020: Approved by the Government of the Russian Federation on April 24, 2012 no. 1853p-P8. Accessed April 17, 2019.

  2. 2

    Gosudarstvennyi reestr selektsionnykh dostizhenii, dopushchennykh k ispol’zovaniyu (The State Register of Breeding Achievements Allowed for Use), vol. 1: Sorta rastenii (Plant Cultivars), Moscow, 2018.

  3. 3

    Klykov, A.G., Parskaya, N.S., Chaykina, E.L., and Anisimov, M.M., Productivity and Quality of Fagopyrum esculentum Moench Cultivars in the Conditions of Primorye Krai, Russ. Agric. Sci., 2018, vol. 44, no. 4, pp. 295–299.

    Article  Google Scholar 

  4. 4

    Maksymiec, W., Effect of copper on cellular processes in higher plants, Photosynthetica, 1997, vol. 34, pp. 132–342.

    Article  Google Scholar 

  5. 5

    Gebhart, E., Chromosome damage in individuals exposed to heavy metals, Toxicol. Environ. Chem., 1984, vol. 8, pp. 253–266.

    Article  Google Scholar 

  6. 6

    Tsmokalyuk, N.M., Assessment of the influence of soil pollution by heavy metals on the development and productivity of soybeans in the conditions of the Ussuri district of Primorsky krai, Extended Abstract of Cand. Sci. (Agric.) Dissertation, Vladivostok, 2004.

  7. 7

    Bessonova, V.P., The state of pollen as an indicator of environmental pollution by heavy metals, Ekologiya, 1992, no. 4, pp. 45–50.

  8. 8

    Barsukova, V.S., Fiziologo-geneticheskie aspekty ustoichivosti rastenii k tyazhelym metallam: Analit. obzor (Physiological and Genetic Aspects of Plant Resistance to Heavy Metals: Analytical Overview), Novosibirsk, 1997.

  9. 9

    Sergeeva, L.E. and Bronnikova, L.I., Cell selection with heavy metal ions: New aspects of complex resistance, X Mezhdunarodnaya konferentsiya “Biologiya kletok rastenii in vitro i biotekhnologiya”: Sb. tez. (Proc. X Int. Conf. Plant Cell Biology in vitro and Biotechnology), Kazan, 2013, pp. 82–83.

  10. 10

    Barsukova, E.N., Cell selection of common buckwheat under ionic stress, Agrarn. Ross., 2013, no. 10, pp. 2–4.

  11. 11

    Klykov, A.G., Moiseenko, L.M., and Barsukova, E.N., Biological resources and selection value of species of Fagopyrum Mill., in Molecular Breeding and Nutritional Aspects of Buckwheat, Elsevier, Academic Press, 2016, pp. 51–60.

    Google Scholar 

  12. 12

    Zaprometov, M.N., Fenol’nye soedineniya: Rasprostranenie, metabolizm i funktsii v rasteniyakh (Phenolic Compounds: Distribution, Metabolism, and Functions in Plants), Moscow: Nauka, 1993.

  13. 13

    Goncharenko, A.A., Adaptability and environmental resistance of cereal varieties, Vestn. Ross. S-kh. Akad., 2005, no. 6, pp. 49–53.

  14. 14

    Pakudin, V.Z., Parameters for assessing the ecological plasticity of varieties and hybrids, in Teoriya otbora v populyatsiyakh rastenii (Theory of Selection in Plant Populations), Novosibirsk, 1976, pp. 178–189.

  15. 15

    Barsukova, E.N., RF Patent 2538167, Byull., 2015, no. 1.

  16. 16

    Kalashnikova, E.A., Biological fundamentals of plant cell selection, Dokl. Timiryazevsk. S-kh. Akad., 2003, no. 275, pp. 110–112.

  17. 17

    Pasqualini, V., Robles, C., Garzino, S., Bousquet-Melou, A., and Bonin, G., Phenolic compounds content in Pinushalepensis Mill. Needles: A bioindicator of air pollution, Chemosphere, 2003, vol. 52, pp. 239–248.

    CAS  Article  Google Scholar 

  18. 18

    Kostyuk, V.A. and Potapovich, A.I., Bioradikaly i bioantioksidanty (Bioradicals and Bioantioxidants), Minsk: Belarus. Gos. Univ., 2004.

  19. 19

    Drozdov, S.N., Eremin, G.V., Klimashevskii, E.L., et al., Diagnostika ustoichivosti rastenii k stressovym vozdeistviyam: Metod. rukovodstvo (Diagnostics of Plant Resistance to Stress: Methodological Manual), Leningrad: VIR, 1988.

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The study was carried out within the framework of the complex program of fundamental research of the Far East Branch of the Russian Academy of Science for 2018–2020 (project no. 18-5-025).

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Correspondence to A. G. Klykov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by N. Statsyuk

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Barsukova, E.N., Klykov, A.G. & Chaikina, E.L. Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench. Russ. Agricult. Sci. 45, 503–506 (2019).

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  • buckwheat
  • Fagopyrum esculentum Moench
  • tissue culture
  • selective factor
  • copper and zinc ions
  • regenerant
  • tolerance
  • breeding
  • rutin
  • large grain size
  • productivity
  • adaptability