A tissue culture method has been used for the breeding of buckwheat (Fagopyrum esculentum Moench) in the presence of high concentrations of heavy metal ions in a nutrient medium as selective factors. Based on the Izumrud (Russia) and Kitawase (Japan) varieties and their hybrid (Izumrud × Kitawase), some copper- and zinc-tolerant regenerant lines have been derived. The negative effect of copper ions in the form of chlorophyll mutants has been observed starting from the second generation of regenerant plants. A positive effect of heavy metals on the increase of a rutin content in regenerants has been observed under in vitro conditions. The field evaluation of heavy metal-tolerant samples has allowed us to select lines highly adapted to the monsoon climate of Primorsky krai and to combine such features as large grain size (thousand kernel weight is equal to 36–38 g), high seed productivity (1.9–3.2 g), and high rutin content in green mass (2.8–4.3%).
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The study was carried out within the framework of the complex program of fundamental research of the Far East Branch of the Russian Academy of Science for 2018–2020 (project no. 18-5-025).
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Translated by N. Statsyuk
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Barsukova, E.N., Klykov, A.G. & Chaikina, E.L. Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench. Russ. Agricult. Sci. 45, 503–506 (2019). https://doi.org/10.3103/S1068367419060028
- Fagopyrum esculentum Moench
- tissue culture
- selective factor
- copper and zinc ions
- large grain size