Skip to main content

Advertisement

Springer Nature Link
Log in

Variability of Rice Haploids Obtained From In Vitro Anther Culture

  • CROP PRODUCTION
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

Morphological variability of haploid plants and doubled haploids of rice obtained from one callus line in in vitro anther culture has been studied. The study was performed on rice (Oryza sativa L. subsp. japonica Kato) plants of the Kaskad varietу. Regenerants of the same callus line derived from one rice anther (four lines in total) were divided into 2–3 groups of 20–30 plants (depending on the sample size) in the order of their differentiation on a callus and transplantation on the rooting medium. Two callus lines (15.1 and 18.1) generated both haploids and doubled haploids (in equal proportion), while two other callus lines (5.1 and 7.2) formed numerous haploids. In the case of the 5.1 and 7.2 lines, regenerants with a larger ordinal number were characterized by decreased size indices of derived plants (plant height, number of flowers on the main panicle, and the number of panicles). No statistically significant difference has been revealed between the haploids and doubled haploids originating from the 15.1 and 18.1 lines. To induce the doubling of a chromosome number in haploid regenerants by colchicine or other antitubulin compounds, it is recommended to use the earliest plants developing on a callus. Using the Hotelling’s T2 criterion calculated for the whole complex of biometric indices, a statistically significant (p = 0.001) difference was revealed between haploids of four callus lines and doubled haploids of two callus lines. Haploids of different lines differed by 3–4 traits, while doubled haploids differed by three of five traits (panicle length, productive tilling capacity, and plant height). Rice varieties interesting for breeders may be improved using in vitro anther cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

REFERENCES

  1. Mishra, R. and Rao, G.J.N., In-vitro androgenesis in rice: Adventages, constraints and future prospects, Rice Sci., 2016, vol. 23, no. 2, pp. 57–68.

    Article  Google Scholar 

  2. Germana, M.A., Gametic embryogenesis and haploid technology as valuable support to plant breeding, Plant Cell Rep., 2011, vol. 30, pp. 839–857.

    Article  CAS  PubMed  Google Scholar 

  3. Datta, S.K., Androgenic haploids: Factors controlling development and its application in crop improvement, Curr. Sci., 2005, vol. 89, no. 11, pp. 1870–1878.

    CAS  Google Scholar 

  4. Dunwell, J.M., Haploids in flowering plants: Origins and exploitation, Plant Biotechnol. J., 2010, vol. 8, pp. 377–424.

    Article  CAS  PubMed  Google Scholar 

  5. Kucherenko, L.A. and Kharchenko, P.N., Using the method of tissue culture in rice breeding, Sel’sk. Khoz. Rubezhom, 1975, no. 11, pp. 20–21.

  6. Kruglova, N.N., Innovative biotechnology of spring soft wheat androclinic haploidy: An embryological approach, Agrar. Ross., 2009, no. 1, pp. 34–38.

  7. Zagorska, N.A., Shtereva, L.A., Kruleva, M.M., et al., Induced androgenesis in tomato (Lycopersicon esculentum Mill.). III. Characterization of the regenerants, Plant Cell Rep., 2004, vol. 22, pp. 449–456.

    Article  CAS  PubMed  Google Scholar 

  8. Alermanno, L. and Guiderdoni, E., Increased doubled haploid plant regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media, Plant Cell Rep., 1994, vol. 13, pp. 432–436.

    Google Scholar 

  9. Rout, P., Naik, N., Ngangkham, U., et al., Doubled haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization, Plant Biotechnol., 2016, vol. 33, pp. 177–186.

    Article  CAS  Google Scholar 

  10. Mishra, R., Rao, G.J.N., Rao, R.N., et al., Development and characterization of elite doubled haploid lines from two Indica rice hybrids, Rice Sci., 2015, vol. 22, no. 6, pp. 290–299.

    Article  Google Scholar 

  11. Pauk, J., Puolimatka, M., Toth, K.L., and Monostori, T., In vitro androgenesis of triticale in isolated microspore culture, Plant Cell Tissue Organ Cult., 2000, vol. 61, pp. 221–229.

    Article  CAS  Google Scholar 

  12. Cistue, L., Soriano, M., Castillo, A.M., et al., Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture, Plant Cell Rep., 2006, vol. 25, pp. 257–264.

    Article  CAS  PubMed  Google Scholar 

  13. Kang, T.-J., Yang, M.-S., and Deckard, E., The effect of osmotic potential on anther culture in spring wheat (Triticum aestivum), Plant Cell Tissue Organ Cult., 2003, vol. 75, pp. 35–40.

    Article  CAS  Google Scholar 

  14. Kasha, K.J., Hu, T.C., Oro, R., et al., Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores, J. Exp. Bot., 2001, vol. 52, no. 359, pp. 1227–1238.

    CAS  PubMed  Google Scholar 

  15. Ilyushko, M.V., Skaptsov, M.V., and Romashova, M.V., Nuclear DNA content in rice regenerants obtained in anther culture in vitro, Sel’skokhoz. Biol., 2018, vol. 53, no. 3, pp. 531–538.

    Google Scholar 

  16. Maletskii, S.I., Yudanova, S.S., and Maletskaya, E.I., Epigenomic and epiplastomic variability in haploid and dihaploid sugar beet plants (Beta vulgaris L.), Sel’skokhoz. Biol., 2015, vol. 50, pp. 579–589.

    Google Scholar 

  17. Sibikeeva, Yu.E. and Sibikeev, S.N., The influence of combinations of alien translocations on in vitro androgenesis in near-isogenic lines of spring bread wheat, Russ. J. Genet., 2014, vol. 50, no. 7, pp. 728–735.

    Article  CAS  Google Scholar 

  18. Heberle-Bors, E., In vitro haploid formation from pollen: A critical review, Theor. Appl. Genet., 1985, vol. 71, pp. 361–374.

    Article  CAS  PubMed  Google Scholar 

  19. Ilyushko, M.V. and Romashova, M.V., The regenerative ability of callus rice transplants in the anther culture in vitro, Agrar. Vestn. Primor’ya, 2018, no. 1, pp. 5–8.

  20. Goncharova, Yu.K., Ispol’zovanie metoda kul’tury pyl’nikov v selektsii risa (Application of the Anther Culture Method in Rice Breeding), Krasnodar: VNII risa, 2012.

  21. Khokhlov, S.S., Tyrnov, V.S., Grishina, E.V., et al., Gaploidiya i selektsiya (Haploidy and Selection), Moscow: Nauka, 1976.

Download references

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Author information

Authors and Affiliations

  1. Far East Federal Research Center of Agrobiotechnology, 692539, pos. Timiryazevsky, Primorsky krai, Russia

    M. V. Ilyushko & M. V. Romashova

Authors
  1. M. V. Ilyushko
    View author publications

    Search author on:PubMed Google Scholar

  2. M. V. Romashova
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to M. V. Ilyushko.

Additional information

Translated by N. Statsyuk

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ilyushko, M.V., Romashova, M.V. Variability of Rice Haploids Obtained From In Vitro Anther Culture. Russ. Agricult. Sci. 45, 243–246 (2019). https://doi.org/10.3103/S1068367419030108

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S1068367419030108

Keywords: