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Genetic Diversity of Barley Accessions from Ethiopia for Powdery Mildew Resistance

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Abstract

In laboratory experiments, 925 Ethiopian barley accessions were studied for juvenile resistance to the northwest population of powdery mildew causative agent Blumeria graminis (DC.) Golovin ex Speer f. sp. hordei Marchal. Based on phenotypic screening, the 27 accessions resistant to the pathogen were selected and 47 forms were heterogenous for the studied character. The resistant and susceptible plants of the examined accessions were analyzed with the use of molecular markers developed for identification of the mlo11 allele. Fifteen accessions carrying the mlo11 allele, which determines durable resistance of most modern barley varieties to powdery mildew, were revealed. The accessions differed by the resistance expression that can be explained both by the manifestation of other resistance genes, which are inadequate to the mlo11, and also by the presence of different mlo11 allelic variants in the genotypes of the selected accessions. The resistance to B. graminis of the other 59 forms is controlled by the effective genes that are different from mlo11.

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REFERENCES

  1. Vavilov, N.I., Proiskhozhdenie i geografiya kul’turnykh rastenii (The Origin and Geography of Cultivated Plants), Leningrad: Nauka, 1987.

  2. Bonman, J.M., Bockelman, H.E., Jackson, L.F., and Steffenson, B.J., Disease and insect resistance in cultivated barley accessions from the USDA National Small Grains Collection, Crop Sci., 2005, vol. 45, no. 4, pp. 1271–1280.

    Article  Google Scholar 

  3. Konovalova, G.S. and Soboleva, O.N., Sources of resistance in barley from Southeast Asian to the causative agent of rhinosporiosis (Rhynchosporium secalis), Mikol. Fitopatol., 2010, vol. 44, no. 3, pp. 248–254.

    Google Scholar 

  4. Khokhlova, A.P., Barley resistance to head smut and composition of genes controlling this trait, Cand. Sci. (Agric.) Dissertation, Leningrad: Vavilov Institute of Plant Industry, 1990.

  5. Weibull, J., Walther, U., Sato, K., Habekuß, A., Kopahnke, D., and Proeseler, G., Diversity in resistance to biotic stresses, in Diversity in Barley (Hordeum vulgare), Elsevier, 2003, pp. 143–178.

    Google Scholar 

  6. Reinstädler, A., Müller, J., Jerzy, H., Czembor, J.H., Piffanelli, P., and Panstruga, R., Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein, BMC Plant Biol., 2010, vol. 10. https://doi.org/10.1186/1471-2229-10-31

  7. Iovieno, P., Andolfo, G., Schiavulli, A., Catalano, D., Ricciardi, L., Frusciante, L., Raffaella, M., and Pavan, S., Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp, BMC Genomics, 2015, vol. 16. https://doi.org/10.1186/s12864-015-2325-3

  8. Büschges, R., Hollricher, K., Panstruga, R., Simons, G., Wolter, M., Frijters, A., Van Daelen, R., Van der Lee, T., Diergaarde, P., Groenendijk, J., Topsch, S., Vos, P., Salamini, F., and Schulze-Lefert, P., The barley Mlo gene: A novel control element of plant pathogen resistance, Cell, 1997, vol. 88, no. 5, pp. 695–705.

    Article  PubMed  Google Scholar 

  9. Wolter, M., Hollricher, K., Salamini, F., and Schulze-Lefert, P., The mlo resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defense mimic phenotype, Mol. Gen. Genet., 1993, vol. 239, nos. 1–2, pp. 122–128. https://doi.org/10.1007/BF00281610

    Article  CAS  PubMed  Google Scholar 

  10. Dreiseitl, A., Frequency of powdery mildew resistances in spring barley cultivars in Czech variety trials, Plant Prot. Sci., 2012, vol. 48, no. 1, pp. 17–20. https://doi.org/10.17221/11/2011-PPS

    Article  Google Scholar 

  11. Piffanelli, P., Ramsay, L., Waugh, R., Benabdelmouna, A., D’Hont, A., Hollricher, K., Jørgensen, J.H., Schulze-Lefert, P., and Panstruga, R., A barley cultivation-associated polymorphism conveys resistance to powdery mildew, Nature, 2004, vol. 430, no. 7002, pp. 887–891.

    Article  CAS  PubMed  Google Scholar 

  12. Ge, X., Deng, W., Lee, Z.Z., Lopez-Ruiz, F.J., Schweizer, P., and Ellwood, S.R., Tempered mlo broad-spectrum resistance to barley powdery mildew in an Ethiopian landrace, Sci. Rep., 2016, vol. 6. https://doi.org/10.1038/srep29558

  13. Alpat'eva, N.V., Abdullaev, R.A., Anisimova, I.N., Gubareva, N.K., Kovaleva, O.N., and Radchenko, E.E., Local barley samples from Ethiopia resistant to powdery mildew, Tr. Prikl. Bot., Genet. Sel., 2016, vol. 177, no. 4, pp. 70–78.

    Google Scholar 

  14. Mains, E.B. and Dietz, S.M., Physiologic forms of barley mildew Erysiphe graminis DC, Phytopathology, 1930, vol. 20, no. 3, pp. 229–239.

    Google Scholar 

  15. Dorokhov, D.B. and Kloke, E., Fast and economical technology of RAPD analysis of plant genomes, Genetika, 1997, vol. 33, no. 4, pp. 443–450.

    Google Scholar 

  16. Anisimova, I.N., Alpat’eva, N.V., and Timofeeva, G.I., Skrining geneticheskikh resursov rastenii s ispol’zovaniem DNK-markerov: Osnovnye printsipy, vydelenie DNK, postanovka PTsR, elektroforez v agaroznom gele. Metodicheskie ukazaniya (Screening of Plant Genetic Resources Using DNA Markers: Basic Principles, DNA Extraction, PCR, and Agarose Gel Electrophoresis. Methodical Instructions), St. Petersburg: Vavilovskii Iinst. Rast., 2010.

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FUNDING

This work was supported by the Russian Foundation for Basic Researches, project no. 18-016-00075.

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Correspondence to R. A. Abdullaev.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Bibov

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Abdullaev, R.A., Lebedeva, T.V., Alpatieva, N.V. et al. Genetic Diversity of Barley Accessions from Ethiopia for Powdery Mildew Resistance. Russ. Agricult. Sci. 45, 232–235 (2019). https://doi.org/10.3103/S1068367419030029

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