Skip to main content
Log in

Perspective Technologies of Isolated Tissue Culture in the Sugar Beet Breeding Process

  • CROP PRODUCTION
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The methods of isolated tissue cultures allowing production and rapid propagation of the sugar beet plant material with improved or new traits are described in the presented study. It was shown that the proposed original scheme of mass reproduction and in vitro deposition of elite plants—components of highly productive hybrids—can be used for the production of seeds with improved quality. The technology of in vitro selective selections carried out under abiotic stress conditions allows for the production of sugar beet isogenic lines with high resistance to external environment abiotic factors (salinization, drought, soil acidity). The effect of certain factors of in vitro culture on haploid cells of sugar beet ovules allowed production of doubled haploid lines (DH) with high homozygosity level and qualitative seeds of highly productive hybrid components. The developed technologies based on biotechnological methods are a preferred and innovative direction of research in modern science, since the introduction of these developments in the breeding process will contribute to the production of competitive hybrids with a set of desired economic traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Znamenskaya, V.V., In vitro microcloning as a method for maintaining and reproducing sugar beet lines, in Entsiklopediya roda Beta. Biologiya, genetika i selektsiya svekly (nauch. tr. Instituta tsitologii i genetiki SO RAN) (Encyclopedia of the Beta Genus. Biology, Genetics, and Breeding of Beets (Scientific Proceedings of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences)), Novosibirsk, 2010, pp. 420–437.

  2. Oshevnev, V.P. and Gribanova, N.P., Selection of self-compatible O-type pollinators in sugar beet, in Entsiklopediya roda Beta. Biologiya, genetika i selektsiya svekly (nauch. tr. Instituta tsitologii i genetiki SO RAN) (Encyclopedia of the Beta Genus. Biology, Genetics, and Breeding of Beets (Scientific Proceedings of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences)), Novosibirsk: Sova, 2010, pp. 542–554.

  3. Oshevnev, V.P., Gribanova, N.P., Kolosova, N.N., Samodurova, N.I., and Novikova, L.N., Izuchenie genofonda linii sakharnoy svekly na geterozis po urozhaynosti i sakharistosti korneplodov (Study of the Gene Pool of Sugar Beet Lineages for Heterosis by Yield and Sugar Content of Root Crops), Voronezh: Voronezhskii TsNTI, 2014, pp. 3–9.

  4. Zhuzhzhalova, T.P., Znamenskaya, V.V., Oshevnev, V.P., Gribanova, N.P., Kolesnikova, E.O., Vasil’chenko, E.N., and Cherkasova, N.N., Innovative in vitro microcloning of sugar beet in the breeding process, Sakh. Svekla, 2017, no. 4, pp. 12–18.

  5. Dukhovskii, P., Plant response to the complex effect of natural and anthropogenic stress, Fiziol. Rast., 2003, pp. 165–173.

  6. Cherkasova, N.N. and Zhuzhzhalova, T.P., Obtaining osmous-resistant sugar beet plants in an in vitro culture, Sakh. Svekla, 2011, no. 7, pp. 22–24.

  7. Soshinkova, T.N., Radyukina, N.L., Korolkova, D.V., and Nosov, A.V., Proline and functioning of the antioxidant system in Thellungiella salsuginea plants and cultured cells subjected to oxidative stress, Russ. J. Plant Physiol., 2013, vol. 60, no. 1, pp. 41–54.

    Article  CAS  Google Scholar 

  8. Kuznetsov, V.V. and Shevyakova, N.I., Proline under stress: Biological role, metabolism, and regulation, Fiziol. Rast., 1999, no. 2, pp. 321–336.

  9. Lisitsyn, E.M., Potential aluminum resistance of agricultural plants and its implementation in the European Northeast of Russia, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow, 2005.

  10. Vasil’chenko, E.N., Zhuzhzhalova, T.P., Vashchenko, T.G., and Kolesnikova, E.O., Technology of creating restorative lines of sugar beet, Vestn. Voronezh. Gos. Agrarn. Univ., 2018, no. 1, pp. 42–50.

  11. Batygina, T.B., Kruglova, N.N., Gorbunova, V.Yu., Titova, G.E., and Sel’dimirova, O.A., Ot mikrospory—k sortu (From Microspores to Cultivars), Moscow: Nauka, 2010.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to T. P. Zhuzhzhalova.

Additional information

Translated by V. Mittova

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhuzhzhalova, T.P., Kolesnikova, E.O., Cherkasova, N.N. et al. Perspective Technologies of Isolated Tissue Culture in the Sugar Beet Breeding Process. Russ. Agricult. Sci. 45, 21–25 (2019). https://doi.org/10.3103/S106836741901021X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S106836741901021X

Keywords:

Navigation