Russian Agricultural Sciences

, Volume 45, Issue 1, pp 5–12 | Cite as

Biochemical Responses of Wheat Cultivars to PEG-Induced Drought Stress

  • S. S. DatirEmail author
  • A. Inamdar


Aim of the present study was to study the biochemical responses of wheat cultivars (Platinum Lok 1, Eagle 135, Prerna Amber, Kisan Farmer 2189 and Ankur Kedar) to polyethylene glycol (PEG-6000—0, 5, 10 and 15%) induced drought stress. Lipid peroxidation, H2O2, proline, glycine betaine (GB) and activities of the antioxidant enzymes were evaluated at seedling stage. As the concentration of PEG increased, an increase in free proline and GB accumulation was observed in all the wheat cultivars to different extent. However, the magnitude of reduction of these parameters was more in Prerna Amber and Eagle 135. Platinum Lok 1 showed an increase in free proline and GB contents with low levels of MDA and H2O2 contents as compared to rest of the cultivars. The activities of catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD) enzymes showed increasing trend with respect to increasing PEG concentration. However, no specific trend was observed for the activity of ascorbate peroxidase (APX). Activities of POX and SOD were significantly high in Plantinum Lok 1. Overall results indicated that Platinum Lok 1 can be considered as drought tolerant which can be associated with higher proline and GB accumulation and lower MDA and H2O2 contents. Biochemical parameters such as osmolytes and antioxidant enzymes could provide useful tools for identification of drought tolerant wheat cultivars at seedling stage.


antioxidant enzymes drought glycine betaine proline wheat 



The research was supported by Department Research and Development Program (DRDP) funds.

Authors are also thankful to Prof. Ameeta Ravikumar, HoD, Department of Biotechnology, Savitribai Phule Pune University, Pune for her kind support and providing laboratory facilities during this work.


  1. 1.
    Farooq, M., Hussain, M., Wahid, A., and Siddique, K.H.M., Drought Stress in Plants: An Overview, Springer-Verlag Berlin Heidelberg, 2012.Google Scholar
  2. 2.
    Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., and Basra, S.M.A., Plant drought stress: Effects, mechanisms and management, Agron. Sustain. Dev., 2009, vol. 29, pp. 185–212.CrossRefGoogle Scholar
  3. 3.
    Demirevska, K., Zasheva, D., Dimitrov, R., Simova-Stoilova, L., Stamenova, M., and Feller, U., Drought stress effects on Rubisco in wheat: Changes in the Rubisco large subunit, Acta Physiol. Plant., 2009, vol. 31, pp. 1129–1138.CrossRefGoogle Scholar
  4. 4.
    Kaur, S., Gupta, A.K., and Kaur, N., Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea, J. Agron. Crop Sci., 2005, vol. 191, pp. 81–87.CrossRefGoogle Scholar
  5. 5.
    Kumar, S., Beena, A.S., Awana, M., and Singh, A., Salt-induced tissue-specific cytosine methylation downregulates expression of HKT genes in contrasting wheat (Triticum aestivum L.) genotypes, DNA Cell Biol., 2017, vol. 36, pp. 283–394.CrossRefGoogle Scholar
  6. 6.
    Kajla, M., Yadav, V.K., Khokhar, J., Singh, S., Chhokar, R.S., Meena, R.P., and Sharma, R.K., Increase in wheat production through management of abiotic stresses: A review, J. App. Nat. Sci., 2015, vol. 7, pp. 1070–1080.CrossRefGoogle Scholar
  7. 7.
    Singh, A., Yadav, O.P., Gaikwad, K., Kumar, S., and Rai, R.D., Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition, Ind. J. Biochem. Biophys., 2015, vol. 52, pp. 75–85.Google Scholar
  8. 8.
    Ashraf, M. and Foolad, M.R., Roles of glycine betaine and proline in improving plant abiotic stress resistance, Environ. Exp. Bot., 2007, vol. 59, pp. 206–216.CrossRefGoogle Scholar
  9. 9.
    Tomar, S.M.S. and Kumar, G.T., Seedling survivability as a selection criterion for drought tolerance in wheat, Plant Breed., 2004, vol. 123, pp. 392–394.CrossRefGoogle Scholar
  10. 10.
    Ahmad, M., Shabbir, G., Minhas, N.M., and Shah, M.K.N., Identification of drought tolerant wheat genotypes based on seedling traits, Sarhad. J. Agric., 2013, vol. 29, pp. 21–27.Google Scholar
  11. 11.
    Carpita, N., Sabularse, D., Mofezinos, A., and Dekmer, D., Determination of the pore size of cell walls of living plant cells, J. Sci., 1979, vol. 205, pp. 1144–1147.CrossRefGoogle Scholar
  12. 12.
    Chachar, Z., Chachar, N.A., Chachar, Q.I., Zujtaba, S.M., Chachar, G.A., and Chachar, S., Identification of drought tolerant wheat genotypes under water deficit conditions, Int. J. Res. Granth., 2016, vol. 4, pp. 204–214.Google Scholar
  13. 13.
    Murashige, T. and Skoog, F., A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiol. Plant., 1962, vol. 15, pp. 473–497.CrossRefGoogle Scholar
  14. 14.
    Bates, L., Rapid determination of free proline for water stress studies, J. Plant Soil., 1973, vol. 39, pp. 205–207.CrossRefGoogle Scholar
  15. 15.
    Grieve, C. and Grattan, S., Rapid assay for determination of water soluble quaternary amino compounds, J. Plant Soil., 1983, vol. 70, pp. 303–307.CrossRefGoogle Scholar
  16. 16.
    Heath, R.L. and Packer, L., Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, pp. 189–198.CrossRefGoogle Scholar
  17. 17.
    Velikova, V., Yondanov, I., and Edreva, A., Oxidative stress and some antioxidant system in acid rain treated bean plants. Protection role of exogenous polyamines, J. Plant Sci., 2000, vol. 151, pp. 59–66.CrossRefGoogle Scholar
  18. 18.
    Shuaib, M., Khan, I., Ali, Z., and Ali, W., Evaluation of different wheat varieties by SDS PAGE electrophoresis, J. Biol. Sci., 2007, vol. 10, pp. 1667–1672.Google Scholar
  19. 19.
    Chance, S. and Maehly, A., Assay of catalase and peroxidase, Methods Enzymol., 1955, vol. 2, pp. 764–775.CrossRefGoogle Scholar
  20. 20.
    Beauchamp, C. and Fridovich, I., Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels, Anal. Biochem., 1971, vol. 44, pp. 276–287.CrossRefGoogle Scholar
  21. 21.
    Nakano, Y. and Asada, K., Purification of ascorbate peroxidase in spinach chloroplast; its inactivation in ascorbate depleted medium and reactivation by monodehydroascorbate radical, J. Plant Cell Physiol., 1987, vol. 28, pp. 131–140.Google Scholar
  22. 22.
    del Pozo, A., Yanez, A., Matus, I.A., Tapia, G., Castillo, D., Sanchez-Jardon, L., et al., Physiological traits associated with wheat yield potential and performance under water-stress in a Mediterranean environment, Front. Plant Sci., 2016, vol. 7, p. 987.CrossRefGoogle Scholar
  23. 23.
    Basu, S., Roychoudhury, A., Saha, P.P., and Sengupta, D.N., Comparative analysis of some biochemical responses of three indica rice varieties during polyethylene glycol-mediated water stress exhibits distinct varietal differences, Acta. Physiol. Plant., 2010, vol. 32, pp. 551–563.CrossRefGoogle Scholar
  24. 24.
    Chakraborty, U. and Pradhan, B., Oxidative stress in five wheat varieties exposed to water stress and study of their antioxidative enzyme defense system, water stress responsive metabolites and H2O2 accumulation, J. Plant Physiol., 2012, vol. 24, pp. 117–130.Google Scholar
  25. 25.
    Luo, W., Song, F., and Xie, Y., Trade-off between tolerance to drought and tolerance to flooding in three wetland plants, Wetlands, 2008, vol. 28, pp. 866–873.CrossRefGoogle Scholar
  26. 26.
    Ali, Q. and Ashraf, M., Induction of drought tolerance in maize (Zea mays L.) due to exogenous application of trehalose: Growth, photosynthesis, water relations and oxidative defense mechanism, J. Agron. Crop Sci., 2011, vol. 197, pp. 258–271.CrossRefGoogle Scholar
  27. 27.
    Bohnert, H.J. and Jensen, R.G., Strategies for engineering water-stress tolerance in plants, Trends Biotechnol., 1996, vol. 14, pp. 89–97.CrossRefGoogle Scholar
  28. 28.
    Mwadzingeni, L., Shimelis, H., and Tesfay, S., Screening of bread wheat genotypes for drought tolerance using phenotypic and proline analyses, Front. Plant Sci., 2016, vol. 7, pp. 1276.CrossRefGoogle Scholar
  29. 29.
    Szegletes, Zs., Erdei, L., Tari, I., and Cseuz, L., Accumulation of osmoprotectants in wheat cultivars of different drought tolerance, Cereal Res. Commun., 2000, vol. 28, pp. 403–410.Google Scholar
  30. 30.
    Saed-Moucheshi, A., Shekoofa, A., and Pessarakli, M., Reactive oxygen species (ROS) generation and detoxifying in plants, J. Plant Nutr., 2014, vol. 37, pp. 1573–1585.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Savitribai Phule Pune UniversityPuneIndia
  2. 2.Modern College of Agricultural BiotechnologyPuneIndia

Personalised recommendations