Ultrastructural organization of the domains in the cell nucleus of dicotyledonous and monocotyledonous plants under abiotic stress

Abstract

The transformation of the structural organization of interphase nuclei of a plant cell, depending on the type of tissue, ploidy, and the action of abiotic factors of the medium, has been studied by light and transmission electron microscopy. It is shown that the location and the quantitative relationship between condensed and decondensed chromatin, the presence and localization of nuclear bodies in the plant nucleus, and the presence of invaginations of the nuclear membrane and inclusions depend on the tissue type and cell age as well as the intensity, time, and type of exposure to abiotic factors. Examples of different degrees of chromatin condensation in one plant organism are given. It has been established that the degree of chromatin condensation and decondensation, the state of other domains and nonspecific inclusions of the cell nucleus can be artificially modeled for research purposes or subsequent modification. The issue of application of such technologies for the creation and selection of stable forms of agricultural plants taking into account the controlled modification of their genome is discussed. A possible mechanism for incorporation of nonspecific inclusions in a nuclear compartment is proposed.

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Correspondence to E. N. Baranova.

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Original Russian Text © E.N. Baranova, I.A. Chaban, N.V. Kononenko, M.R. Khaliluev, N.K. Christov, A.A. Gulevich, E.G. Todorovska, 2017, published in Rossiiskaya Sel’skokhozyaistvennaya Nauka, 2017, No. 2, pp. 3–10.

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Baranova, E.N., Chaban, I.A., Kononenko, N.V. et al. Ultrastructural organization of the domains in the cell nucleus of dicotyledonous and monocotyledonous plants under abiotic stress. Russ. Agricult. Sci. 43, 199–206 (2017). https://doi.org/10.3103/S1068367417030041

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Keywords

  • interphase nucleus
  • nucleolus
  • euchromatin
  • heterochromatin
  • nuclear bodies
  • nonspecific inclusions
  • condensation and decondensation of chromatin
  • abiotic stress
  • ploidy
  • plant cell
  • ultrastructure