Skip to main content
Log in

Interactive effects of UV-B radiation and salicylic acid on Vigna radiata L.

  • Crop Research
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The present study was conducted to investigate the physiological responses of Vigna radiata L. to UV-B radiation and salicylic acid (SA) alone and in combination. The exposure of the seedlings to UV-B radiation significantly reduced biophysical parameters. The pigments viz., chlorophylls and carotenoids and protein decreased while sugar content increased in the seedlings on UV-B exposure. UV-B stress increased the activities of superoxide dismutase, catalase and peroxidase. SA appears to mitigate the adverse effect of UV-B radiation on the growth and metabolism of Vigna seedlings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Amin, A.A., El-Kader, A.A.A., Shalaby, M.A.F., Gharib, F.A.E., Rashad, E.S.M., and Silva, J.A.T, Physiological effects of salicylic acid and thiourea on growth and productivity of maize plants in sandy soil, Commun. Soil Sci. Plant Anal., 2013, vol. 44, pp. 1141–1155.

    Article  CAS  Google Scholar 

  2. Balakumar, T., Selvakumar, V., and Sathiameena, K, Murugu Ilanchezhian, C., and Paliwal, K., UV-B radiation mediated alterations in the nitrate assimilation pathway of crop plants kinetic characteristics of nitrate reductase, Photosynthetica, 1999a, vol. 37, pp. 459–467.

    Article  CAS  Google Scholar 

  3. Beyer, W.F. and Fridovich, I, Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions, Anal. Biochem., 1987, vol. 161, pp. 559–566.

    Article  CAS  PubMed  Google Scholar 

  4. Bornman, J.F. and Sundby-Emmanuelson, C., Response of plants to UV-B radiation: Some biochemical and physiological effects, Environment and Plant Metabolism, Smirnoff, N., Ed., Oxford: Bioscientific, 1995.

  5. Cakmak, I. and Marschner, H, Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves, Plant Physiol., 1992, vol. 98, pp. 1222–1227.

    CAS  PubMed  Google Scholar 

  6. Caldwell, M.M., Ballare, C.L., Bornman, J.F., Flint, S.D., Bjorn, L.O., Teramura, A.H., Kulandaivelu, G., and Tevini, M, Terrestrial ecosystem increased solar ultraviolet radiation and interaction with other climatic change factor, Photochem. Photobiol. Sci., 2003, vol. 2, pp. 29–38.

    Article  CAS  PubMed  Google Scholar 

  7. Caldwell, M.M., Teramura, A.H., Tevini, M., Bornman, J.F., Bjorn, L.O., and Kulandaivelu, G, Effects of increased solar ultraviolet radiation on terrestrial plants, AMBIO, 1995, vol. 24, pp. 166–173.

    Google Scholar 

  8. El-Tayeb, M.A, Differential response of two Vicia faba cultivars to drought: Growth,pigments,lipid peroxidation, organic solutes, catalase and peroxidase activity, Acta Agron. Hung., 2006, vol. 54, pp. 25–37.

    Article  CAS  Google Scholar 

  9. Geeta, A.K.G, Comparative study of IRand UV-B radiation exposure on germination,biomass and chlorophyll content of Pisum sativum (L.), Int. J. Sci. Res., 2014, vol. 3, no. 11, pp. 2616–2619.

    Google Scholar 

  10. Gill, P.K., Sharma, A.D., Singh, P., and Bhullar, S.S, Effect of various abiotic stresses on the growth, soluble sugars and water relations of sorghum seedlings grown in light and darkness, Bulg. J. Plant Physiol., 2001, vol. 27, nos. 1–2, pp. 72–84.

    CAS  Google Scholar 

  11. Hedge, J.E. and Hofreiter, B.T., Estimation of carbohydrate, Methods in Carbohydrate Chemistry, Whistler, R.L. and BeMiller, J.N., Eds., New York: Academic Press, 1962, pp. 17–22.

  12. Hemeda, H.M. and Klein, B.P, Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts, J. Food Sci., 1990, vol. 55, pp. 184–185.

    Article  CAS  Google Scholar 

  13. Hoagland, D.R. and Arnon, D.I, The water culture method for growing plants without soil, in California Agriculture Experiment Station, Circular, Berkeley, CA: University of California, 1950.

    Google Scholar 

  14. Hybner, R.M., Peas: P. sativum. An introduced legume for conservation use in Montana and Wyoming, Montana: United States Department of Agriculture and Natural Resources Conservation Service, 2014.

    Google Scholar 

  15. Jaworski, E, Nitrate reductase assay in intact plant tissue, Biochem. Biophys. Res., 1971, vol. 43, pp. 1274–1279.

    Article  CAS  Google Scholar 

  16. Kaplan, F. and Guy, C.L., Beta-amylase induction and the protective role of maltose during temperature shock, Plant Physiol., 2004, vol. 135, pp. 1674–1684.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Lichtenthaler. H.K., Chlorophyll and carotenoids: Pigments of photosynthetic bio-membranes, Methods in Enzymology, Packer, L. and Douce, R., Eds., San Diego: Academic Press, 1987, pp. 350–382.

  18. Lowry, O.H., Rosenbrough, R.J., Farr, A.L., and Randall, R.J, Protein measurement with folin phenol reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  PubMed  Google Scholar 

  19. Martel, A.B. and Qaderi, M.M, Does salicylic acid mitigates the adverse effects of temperature and ultraviolet- B radiation on pea (Pisum sativum) plants?, Environ. Exp. Bot., 2016, vol. 122, pp. 39–48.

    Article  CAS  Google Scholar 

  20. Mishra, V., Srivastava, G., and Prasad, S.M, Antioxidant response of bitter gourd (Momordica charantia L.) seedlings to interactive effect of dimethoate and UV-B irradiation, Sci. Hortic., 2009, vol. 120, pp. 373–378.

    Article  CAS  Google Scholar 

  21. Nakano, Y. and Asada, K, Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.

    CAS  Google Scholar 

  22. Santos, I., Fidalgo, F., Almeida, J.M., and Salema, R, Biochemical and ultra structural changes in leaves of potato plants grown under supplementary UV-B radiation, Plant Sci., 2004, vol. 167, pp. 925–935.

    Article  CAS  Google Scholar 

  23. Singh, R., Singh, S., Tripathi, R., and Agrawal, S.B, Supplemental UV-B radiation induced changes in growth, pigments and antioxidant pool of bean (Dolichos lablab) under field conditions, J. Environ. Biol., 2011, vol. 32, pp. 139–145.

    CAS  PubMed  Google Scholar 

  24. Singh N.B., Effect of salicylic acid on cabbage (Brassica oleracea cv. capitata) grown under salinity stress, IJPP, 2014, vol. 4, no. 4, pp. 1109–1118.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to N. B. Singh.

Additional information

The article is published in the original.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bano, C., Singh, N.B. & Sunaina Interactive effects of UV-B radiation and salicylic acid on Vigna radiata L.. Russ. Agricult. Sci. 43, 128–133 (2017). https://doi.org/10.3103/S1068367416060057

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S1068367416060057

Keywords

Navigation