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Agrobacterium-mediated transformation efficiency of carrot embryogenic callus using nptII and hpt selective genes

  • Plant Growing
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Russian Agricultural Sciences Aims and scope

Abstract

Experiments were carried out to compare the efficiency of embryogenic carrot calli transformation using selective genes nptII and hpt. Our data showed that it is more preferable to use kanamycin concentration 100 mg/L and hygromycin 20 mg/L for the selection of transgenic shoots. Under the same conditions, the use of kanamycin yielded a greater number of transgenic shoots. It is, therefore, more preferable to use vector constructs containing gene nptII for genetic transformation of the Nantskaya 4 carrot cultivar.

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Authors and Affiliations

  1. Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow oblast, 142290, Russia

    T. N. Sidorova, D. N. Miroshnichenko & S. V. Dolgov

  2. All-Russia Research Institute of Agricultural Biotechnology, Moscow, 127550, Russia

    D. N. Miroshnichenko & S. V. Dolgov

Authors
  1. T. N. Sidorova
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  2. D. N. Miroshnichenko
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  3. S. V. Dolgov
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Correspondence to T. N. Sidorova.

Additional information

Original Russian Text © T.N. Sidorova, D.N. Miroshnichenko, S.V. Dolgov, 2015, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2015, No. 3, pp. 18–22.

Presented by Academician P. N. Kharchenko

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Sidorova, T.N., Miroshnichenko, D.N. & Dolgov, S.V. Agrobacterium-mediated transformation efficiency of carrot embryogenic callus using nptII and hpt selective genes. Russ. Agricult. Sci. 41, 216–221 (2015). https://doi.org/10.3103/S1068367415040175

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  • DOI: https://doi.org/10.3103/S1068367415040175

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