Abstract
PCR analysis of eight microsatellite loci was performed to obtain individual genetic profiles for four transgenic sugar beet lines with mf2 gene, which encodes bacterial protein from Bacillus thuringiensis. Total DNA extractions from the vegetative tissue were carried out by the modified phenol-chloroform method. The primers Bvv15, 21, 23, 30, 32, 43, 53, and 64 were used (Smulders et al., 2010). The efficiency of using RAPD-markers (PawS 6, 16, and 17) for assessing the polymorphism of given plants and their genotyping was considered.
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Original Russian Text © A.S. Hussein, N.N. Bogacheva, A.A. Nalbandyan, Ye.N. Vasilchenko, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 5, pp. 10–13.
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Hussein, A.S., Bogacheva, N.N., Nalbandyan, A.A. et al. PCR analysis for a transgenic forms of sugar beet with mf2 gene. Russ. Agricult. Sci. 40, 404–407 (2014). https://doi.org/10.3103/S1068367414060111
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DOI: https://doi.org/10.3103/S1068367414060111