Abstract
In the course of the creation of initial breeding material, the response of callus tissue initiated by seven various genotypes to bacterization by methylotrophic bacteria and to the addition of perfluorocarbones having a gas transporting function in/on culture medium was studied t- ley plants. After the introduction of carbogal (5 vol %) and perfluorodecalin (5 vol %) into the medium for the callus proliferation, and also after the combination of perftormethyldekalin “layering” (10 vol %) on the induction medium with callus bacterization by Methylobacterium extorquens, a positive effect of the morphogenesis induction was noted. The expediency of the use of perfluorocarbons in pure form, as well as in combination with bacterization by methylotrophic bacteria, for the induction of morphogenesis was confirmed with data of microscopy about reliable increase in the frequency occurrence of the zones of the secondary differentiation of callus tissue.
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Original Russian Text © I.G. Shirokikh, A.V. Bakulina, G.A. Batalova, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 3, pp. 6–10.
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Shirokikh, I.G., Bakulina, A.V. & Batalova, G.A. Induction of morphogenesis in barley callus culture. Russ. Agricult. Sci. 40, 237–241 (2014). https://doi.org/10.3103/S1068367414040144
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DOI: https://doi.org/10.3103/S1068367414040144