Abstract
The protein spectrum of a somatic extract of Dirofilaria immitis was studied. Twenty protein components of different electrophoretic mobility and molecular weight were identified. Gel chromatography of the D. immitis extract revealed 35 protein fractions, six of which exhibited diagnostic efficacy of ELISA. On the basis of specificity indices, one was used for diagnosing dirofilariasis. The fraction includes protein components with a molecular weight of 19.3 and 21. kDa. The sensitivity and specificity of the enzyme immunoassay was respectively 84.0 and 86.4%.
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Yastreb, V.B., Shestakov, A.M., and Lavrova, N.A., Dirofilariasis of the dogs in Moscow city and Moscow oblast and its prophylactics, Veterinariya, 2005, no. 2, pp. 38–39.
Öncel, T. and Vural, G., Seroprevalence of Dirofilaria immitis in stray dogs in Istanbul and Izmir, Turk. J. Vet. Anim. Sci., 2005, no. 29, pp. 785–789.
Simon, F. and Genchi, C., Heartworm Infection in Humans and Animals, Ediciones Universidad de Salamanca, 2001, p. 210.
Wang, L.C., Canine filarial infection in north Taiwan, Acta. Trop., 1997, vol. 68, no. 1, pp. 115–120.
Beskrovnaya, Yu.G., Dirofilariasis cases in Southern Russia: distribution and diagnostics, Cand. Sci. (Biol.) Dissertation, Moscow, 2009.
Espinoza, S.E., Muro, A.A., Lorente, T.F., et al., Anti-Dirofilaria immtis IgE: seroepidemiology and seasonal evolution in an exposed human population, Trop. Med. Parasitol., 1993b, no. 44, pp. 172–176.
Calvert, C.A. and Rawlings, C.A., Therapy of canine heartworm disease, in Current Veterinary Therapy IX, Small Animal Practice, 1986, pp. 406–419.
Courtney, C.H., Zeng, Q.Y., and Tonelli, Q., Sensitivity and specificity of the CITE heatworm antigen test and a comparison with the Dirochek heatworm antigen test, J. Am. Anim. Hosp. Assoc., 1990, vol. 26, pp. 623–628.
Berezhko, V.K., Khaidarov, K.A., Dakhno, I.S., and Shkurka, E.P., Immunochemical analysis of somatic extract from mature Dirofilaria immitis, in Mater. nauchn. konf. “Teoriya i praktika bor’by s parazitarnymi boleznyami” (Proc. Sci. Conf. “Theory and Practical Measures Against Parasitic Diseases”), Moscow, 2008, no. 9, pp. 69–73.
Laemmli, U.K., Cleavage of structural proteins during the assembly of the assembly of the head of bacteriophage T4, Nature, 1970, vol. 16, no. 1, pp. 7–13.
Scholtens, C.F. and Patton, S., Evaluation of an enzyme-linked immunosorbent assay for occult dirofilariasis in a population of naturally exposed dogs, Am. J. Vet. Res., 1983, vol. 44, no. 5, pp. 861–864.
Buoro, I.B.J. and Atwell, R.B., Heartworm diagnostic kit, Aust. Vet. J., 1983, vol. 13, no. 3, p. 134.
Thilsted, J.P., Whorton, J., Hibbs, C.M., Jilson, G.P., Steece, R., and Stromei, M., Comparison of for serotest for the detection of Dirofilaria immitsis infection in dogs, Am. J. Vet. Res., 1987, vol. 48, no. 5, pp. 837–841.
Derzhvina, T.Yu., Mertesheva, M.A., Shernysheva, M.A., and Chernikova, E.A., Dirofilariasis in people of Tula oblast, Med. Parazitol. Parazitarn. Bolezni, 2010, no. 1, pp. 46–47.
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Original Russian Text © V.K. Berezhko, L.A. Napisanova, A.Yu. Medvedev, K.A. Khaidarov, A.N. Shinkarenko, P.V. Kolesnikov, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 1, pp. 71–74.
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Berezhko, V.K., Napisanova, L.A., Medvedev, A.Y. et al. Physicochemical and immunodiagnostic characteristics of whole and fractionated somatic extract of Dirofilaria immitis . Russ. Agricult. Sci. 40, 160–163 (2014). https://doi.org/10.3103/S1068367414020049
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DOI: https://doi.org/10.3103/S1068367414020049