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Russian Agricultural Sciences

, Volume 39, Issue 3, pp 226–235 | Cite as

Effect of growth regulators and explant on plant regeneration of Solanum lycopersicum L. var. cerasiforme

  • Mahmoud OtroshyEmail author
  • Zahra Khalili
  • Mohammad Ali Ebrahimi
  • Mojtaba Khayam Nekoui
  • Kosar Moradi
Plant Cultivation

Abstract

Tomato serves as a model to introduce agronomically important genes into dicotyledonous crop plants and to develop edible vaccines and produce cost-effective therapeutics. This study has developed an efficient protocol of shoot organogenesis for Lycopersicon esculentum Mill. through using of different types of explants and growth regulators. Generally, all explants responded significantly to presence of BAP. Best shoot regeneration for leaf (100%) was achieved on MS supplemented with BAP (2 mg/L) + IAA (0.1 mg/L), whereas it was recorded on MS supplemented with BAP (2 mg/L) and BAP (2 mg/L) + IAA (0.5 mg/L) for cotyledons (95%). In addition, hypocotyls (77%) showed the best shoot response on MS supplemented BAP (3 mg/L). Highest number of shoots per explant was 13.33, 12.25, 7.94 respectively for hypocotyls, leaves, cotyledons. The best medium for highest length of shoot was in the presence of BAP (3 and 2 mg/L) + IAA (0.1 mg/L) respectively for hypocotyl (45 mm) and leaf (40.50 mm) explants. This parameter was achieved for cotyledons (13.32 and 12.5 mm) on MS medium supplemented with BAP (3 and 2) mg/L + IAA (0.1 mg/L), respectively. The increasing of BAP concentration up to 3 (mg/L) causes shoot length to continue developing, but it fell down in the presence of BAP (4 mg/L) due to the toxic effect of growth regulators accumulation. Root formation took place within 10–14 days after culturing on the rooting media. Best root induction (100%) was observed on MS medium supplemented with IAA (0.1 and 0.2 mg/L). All rooted shoots acclimated in phytotron and then cultivated in the greenhouse.

Keywords

Lycopersicon esculentum Mill. plant regeneration multiple shoot induction micropropagation growth regulators explant source 

Abbreviations

BAP

6-benzylaminopurine

IAA

indole acetic acid

IBA

indole-3-butyric acid

MS

Murashige and Skoog medium

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Copyright information

© Allerton Press, Inc. 2013

Authors and Affiliations

  • Mahmoud Otroshy
    • 1
    Email author
  • Zahra Khalili
    • 1
    • 2
  • Mohammad Ali Ebrahimi
    • 2
  • Mojtaba Khayam Nekoui
    • 3
  • Kosar Moradi
    • 1
  1. 1.Department of Tissue CultureAgricultural Biotechnology Research Institute of Iran (ABRII)Najaf Abad, IsfahanI. R. Iran
  2. 2.Department of Agricultural BiotechnologyPayame Noor UniversityTehranI. R. Iran
  3. 3.Agricultural Biotechnology Research Institute of Iran (ABRII)KarajI. R. Iran

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