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Long-term culturing of boar type a spermatogonia

  • Animal Husbandry
  • Published:
Russian Agricultural Sciences Aims and scope

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Abstract

The conditions for maintaining boar type A spermatogonia in culture are optimized. An RT PCR analysis of mRNA of Nanog, PLZF, POU5F1, and VASA genes with hybridization fluorescence detection showed that the conditions of culturing boar spermatogonial cells affect the expression of these genes.

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Authors and Affiliations

  1. Kovalenko All-Russian Experimental Veterinary Research Institute, Moscow, 109428, Russia

    I. P. Savchenkova, M. V. Polyakova, E. V. Viktorova & K. V. Kuleshov

Authors
  1. I. P. Savchenkova
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  2. M. V. Polyakova
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  3. E. V. Viktorova
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  4. K. V. Kuleshov
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Corresponding author

Correspondence to I. P. Savchenkova.

Additional information

Original Russian Text © I.P. Savchenkova, M.V. Polyakova, E.V. Viktorova, K.V. Kuleshov, 2012, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2012, No. 5, pp. 46–49.

This work was supported by the Russian Foundation for Basic Research, grant 10-04-01471-a.

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Savchenkova, I.P., Polyakova, M.V., Viktorova, E.V. et al. Long-term culturing of boar type a spermatogonia. Russ. Agricult. Sci. 38, 396–399 (2012). https://doi.org/10.3103/S1068367412050163

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  • DOI: https://doi.org/10.3103/S1068367412050163

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