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Identification and differentiation of Rift Valley virus strains by the polymerase chain reaction method and restriction analysis

  • Veterinary Science
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

A safe, highly sensitive, specific, and economic method was developed for early detection of RNA and differentiation of Rift Valley fever (RVF) virus strains (isolates) in samples of organs from sick and suspected animals, virus-containing culture materials, and objects of veterinary inspection by means of two-round PCR and restriction analysis of PCR products with the use of a set of the restriction by endonucleases. The method can be included in the scheme of early diagnosis of disease and monitoring the spread of the virus. Restriction analysis allows revealing unique marker sites of amplificates of different RVF virus strains during their study, identification, and certification.

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Authors and Affiliations

  1. All-Russian Veterinary Virology and Microbiology Research Institute, Vladimir, 601120, Russia

    N. N. Vlasova, V. I. Balysheva, N. I. Zakutskii, I. Yu. Khukhorova, S. D. Kushnir, O. V. Kapustina & A. N. Zhukov

Authors
  1. N. N. Vlasova
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  2. V. I. Balysheva
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  3. N. I. Zakutskii
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  4. I. Yu. Khukhorova
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  5. S. D. Kushnir
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  6. O. V. Kapustina
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  7. A. N. Zhukov
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Corresponding author

Correspondence to N. N. Vlasova.

Additional information

Original Russian Text © N.N. Vlasova, V.I. Balysheva, N.I. Zakutskii, I.Yu. Khukhorova, S.D. Kushnir, O.V. Kapustina, A.N. Zhukov, 2012, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2012, No. 1, pp. 47–50.

This work was supported by the Russian Foundation for Basic Research, grant no. 11-08-010245.

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Vlasova, N.N., Balysheva, V.I., Zakutskii, N.I. et al. Identification and differentiation of Rift Valley virus strains by the polymerase chain reaction method and restriction analysis. Russ. Agricult. Sci. 38, 65–68 (2012). https://doi.org/10.3103/S106836741201020X

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  • DOI: https://doi.org/10.3103/S106836741201020X

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