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Effects of NaCl, Na2SO4 and mannitol on utilization of storage protein and transformation of vacuoles in the cotyledons and seedling roots of alfalfa (Medicago sativa L.)

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Abstract

The effects of NaCl, Na2SO4 and mannitol on utilization of storage protein in the cells of cotyledon mesophyll and root meristem during the seed germination of alfalfa (Medicago sativa L.) on the solutions of these substances at different concentrations and identical osmotic pressure were investigated using the transmission electron microscopy technique. Ultrastructural analysis revealed changes in the conversion of storage vacuoles to central vacuole, as well as changes in the quantity and form of residual protein bodies within vacuoles, depending on the osmotic effect of salt influence. At low concentrations corresponding to osmotic pressure of 202.6 kPa Na2SO4 did not affect the conversion of storage vacuoles to vegetative vacuole and the protein utilization. With increasing of concentration Na2SO4 and mannitol caused the retaining of considerable protein reserves, and mannitol did not disturb the transformation processes of vacuoles. NaCl did not prevent the central vacuole formation and protein utilization in cotyledon cells, but not in the root cells. When the concentration of the salt increased up to the value corresponding to the osmotic pressure of 607.8 kPa, NaCl can retard the storage protein mobilization in the root cells. These results demonstrate the sensitivity of processes of storage protein utilization during seed germination to stress factors caused by salinity.

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Authors and Affiliations

  1. All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya 42, Moscow, 127550, Russia

    E. N. Baranova & A. A. Gulevich

  2. All-Russian Institute of Fodder Research, Russian Academy of Agricultural Sciences, Lugovaya, Moscow Region, Russia

    A. A. Gulevich & N. N. Koslov

  3. Department of Cell Biology and Neuroscience, Montana State University, Bozeman, Montana, 59717, USA

    E. B. Kalinina-Turner

Authors
  1. E. N. Baranova
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  2. A. A. Gulevich
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  3. E. B. Kalinina-Turner
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  4. N. N. Koslov
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Correspondence to E. N. Baranova.

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Baranova, E.N., Gulevich, A.A., Kalinina-Turner, E.B. et al. Effects of NaCl, Na2SO4 and mannitol on utilization of storage protein and transformation of vacuoles in the cotyledons and seedling roots of alfalfa (Medicago sativa L.). Russ. Agricult. Sci. 37, 11–19 (2011). https://doi.org/10.3103/S1068367411010034

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