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Isolated pea anther culture

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Abstract

Callus tissues and green globular embryoids are obtained in a pea anther culture on a medium with 2,4-D after cold (4°C) treatment of buds. In experiments with the use of treating buds with cold (4°C) and anther culture with heat (32 and 35°C), green embryogenic and morphogenetic calli are induced and regenerant plants are obtained. The origin of the regenerants is established by genetic markers.

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Authors and Affiliations

  1. All-Russian Pulse and Groat Crops Research Institute, Orel oblast, 302502, Russia

    S. V. Bobkov

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  1. S. V. Bobkov
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Correspondence to S. V. Bobkov.

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Original Russian Text © S.V. Bobkov, 2010, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2010, No. 6, pp. 19–21.

This work was supported by the Russian Foundation for Basic Research, grant no. 09-04-97516-r_tsentr_a.

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Bobkov, S.V. Isolated pea anther culture. Russ. Agricult. Sci. 36, 413–416 (2010). https://doi.org/10.3103/S1068367410060078

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  • DOI: https://doi.org/10.3103/S1068367410060078

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