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Absorption and fluorescence spectra of polynucleotide complexes with ethidium bromide

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Journal of Contemporary Physics (Armenian Academy of Sciences) Aims and scope

Abstract

We study the ethidium bromide (EtBr) interaction with the poly(dA)-poly(dT) and poly[d(A-T)]-poly[d(A-T)] polynucleotides at 0.02 M ionic strength of solution and pH = 7.2. It was revealed that the EtBr binds to the poly[d(A-T)]-poly[d(A-T)] by three modes: the intercalation, semiintercalation and weak (electrostatic), while with the poly(dA)-poly(dT) by two modes: the intercalation and semi-intercalation. Moreover, EtBr binds to the poly(dA)-poly(dT) cooperatively and to the poly[d(A-T)]-poly[d(A-T)] non-cooperatively.

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Authors and Affiliations

  1. Yerevan State University, Yerevan, Armenia

    P. O. Vardevanyan, A. P. Antonyan, M. A. Parsadanyan & M. V. Minasyants

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  1. P. O. Vardevanyan
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  2. A. P. Antonyan
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  3. M. A. Parsadanyan
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  4. M. V. Minasyants
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Correspondence to P. O. Vardevanyan.

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Original Russian Text © P.O. Vardevanyan, A.P. Antonyan, M.A. Parsadanyan, M.V. Minasyants, 2015, published in Izvestiya NAN Armenii, Fizika, 2015, Vol. 50, No. 4, pp. 511-519.

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Vardevanyan, P.O., Antonyan, A.P., Parsadanyan, M.A. et al. Absorption and fluorescence spectra of polynucleotide complexes with ethidium bromide. J. Contemp. Phys. 50, 381–387 (2015). https://doi.org/10.3103/S1068337215040106

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  • DOI: https://doi.org/10.3103/S1068337215040106

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