Journal of Water Chemistry and Technology

, Volume 40, Issue 6, pp 367–369 | Cite as

Application of Crayfish Astacus Leptodactylus for the Analysis of Water Genotoxicity in the Lake Sevan Basin

  • A. E. SimonyanEmail author
  • A. A. Sargsyan
  • G. G. Hovhannisyan
  • N. S. Badalyan
  • S. H. Minasyan
Biological Methods of Water Treatment


Genotoxicity of water pollution at three sites of the Lake Sevan Basin was estimated by using crayfish Astacus leptodactylus as bioindicator. Levels of DNA damage in hemocytes of crayfish were analyzed using the comet assay. A significant rise of the level of DNA damage in terms of parameters of the percentage DNA content in the comet tail and the Olive tail moment was detected in crayfish inhabiting waters near the villages of Artanish and Tsapatakh (Armenia) as compared to the crayfish from the Masrik River Estuary. A significant positive correlation between the parameter of the Olive tail moment in crayfish and the content of Al, Fe, Cu, and Mo in water has been discovered. The obtained data indicate that crayfish Astacus leptodactylus is a sensitive bioindicator for monitoring of aquatic ecosystems.


genotoxicity water pollution comet assay crayfish 


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  1. 1.
    Has-Schon, E., Bogut, I., and Strelec, I., Arch. Environ. Contam. Toxicol., 2006, vol. 50, pp. 545–551.CrossRefGoogle Scholar
  2. 2.
    Farkas, A., Salanki, J., and Specziar, A., Arch. Environ. Contam. Toxicol., 2002, vol. 43, no. 2, pp. 236–243.CrossRefGoogle Scholar
  3. 3.
    Paris-Palacios, S., Biagianti-Risbourg, S., and Vernet, G., Aquat. Toxicol., 2000, vol. 50, no. 1/2, pp. 109–124.CrossRefGoogle Scholar
  4. 4.
    Vasanthi, A.L., Revathi, P., Mini, J., and Munuswamy, N., Chemosphere, 2013, vol. 91, no. 8, pp. 1156–1164.CrossRefGoogle Scholar
  5. 5.
    Shi, P., Zhou, S., Xiao, H., et al., Environ. Pollut., 2017, vol. 233, pp. 35–44.CrossRefGoogle Scholar
  6. 6.
    Pereira, C.D., Abessa, D.M., Choueri, R.B., et al., Marine Environ. Res., 2014, vol. 96, pp. 118–126.CrossRefGoogle Scholar
  7. 7.
    Jha, A.N., Cheung, V.V., Foulkes, M.E., et al., Mutat. Res., 2000, vol. 464, pp. 213–228.CrossRefGoogle Scholar
  8. 8.
    Vilhena, M.S., Costa, M.L., and Berredo, J.F., Environ. Geochem. Health, 2013, vol. 35, no. 4, pp. 477–494.CrossRefGoogle Scholar
  9. 9.
    Goretti, E., Pallottini, M., Ricciarini, M.I., et al., Sci. Total. Environ., 2016, vol. 559, pp. 339–346.CrossRefGoogle Scholar
  10. 10.
    Brittle, S.W., Paluri, S.L., Foose, D.P., et al., Environ. Sci. Technol., 2016, vol. 50, no. 13, pp. 7056–7065.CrossRefGoogle Scholar
  11. 11.
    Tabrez, S., Shakil, S., Urooj, M., et al., J. Environ. Sci. Health., C., 2011, vol. 29, no. 3, pp. 250–275.CrossRefGoogle Scholar
  12. 12.
    Kim, I.Y. and Hyun, C.K., Ecotoxicol. Environ. Saf., 2006, vol. 64, no. 3, pp. 288–297.CrossRefGoogle Scholar
  13. 13.
    Srut, M., Stambuk, A., Pavlica, M., and Klobucar, G.I., Arh. Hig. Rada Toksikol., 2010, vol. 61, pp. 29–36.CrossRefGoogle Scholar
  14. 14.
    Klobucar, G.I., Malev, O., Srut, M., et al., Chemosphere, 2012, vol. 87, no. 1, pp. 62–67.CrossRefGoogle Scholar
  15. 15.
    Babayan, A., Hakobyan, S., Jenderedjian, K., et al., ILEC Foundation. Otsu Shigyo, 2006, pp. 347–362.Google Scholar
  16. 16.
    Tice, R.R., Agurell, E., Anderson, D., et al., Environ. Mol. Mutagen., 2000, vol. 35, no. 3, pp. 206–221.CrossRefGoogle Scholar
  17. 17.
    Schilderman, P.A., Moonen, E.J., Maas, L.M., et al., Ecotoxicol. Environ. Saf., 1999, vol. 44, no. 3, pp. 241–252.CrossRefGoogle Scholar
  18. 18.
    Díaz, S., Arceo, B., Martínez-Tabche, L., et al., Rev. Biol. Trop., 2015, vol. 63, no. 1, pp. 83–96.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • A. E. Simonyan
    • 1
    • 2
    Email author
  • A. A. Sargsyan
    • 2
  • G. G. Hovhannisyan
    • 2
  • N. S. Badalyan
    • 3
  • S. H. Minasyan
    • 4
  1. 1.Dumanskii Institute of Colloid and Water ChemistryNational Academy of Science of UkraineKyivUkraine
  2. 2.Yerevan State UniversityYerevanArmenia
  3. 3.Research Center of Zoology and HydroecologyNational Academy of Sciences of the Republic of ArmeniaYerevanArmenia
  4. 4.Institute of Chemical PhysicsNational Academy of Sciences of the Republic of ArmeniaYerevanArmenia

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