Skip to main content
SpringerLink
Log in

Effect of Phosphate Ions on the Dianion–Anion Equilibrium of Fluorescein Excited State

  • Published:
Bulletin of the Russian Academy of Sciences: Physics Aims and scope

Abstract

Calibration curves for determining the constant of fluorescein equilibrium in the excited state were obtained by decomposing the emission spectrum into separate dianion and anion contours. It is shown that the photoinduced proton transfer at the step of dianion‒anion dissociation competes with radiative processes, and its efficiency increases with the concentration of phosphate ions acting as proton acceptors. The change in apparent dissociation constant ΔpKapp2 during photoexcitation into the absorption band of the fluorescein dianion (488 nm) was found to be close to limit value ΔpKa = −0.71 determined according to the Förster cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Po, H.N. and Senozan, N.M., J. Chem. Educ., 2001, vol. 78, no. 11, p. 1499.

    Article  Google Scholar 

  2. Edmonds, T.E., Flatters, N.J., Jones, C.F., et al., Talanta, 1988, vol. 35, p. 103.

    Article  Google Scholar 

  3. Valeur, B. and Berberan-Santos, M.N., Molecular Fluorescence: Principles and Applications, Weinheim: Wiley, 2012.

    Book  Google Scholar 

  4. Klonis, N. and Sawyer, W.H., J. Fluoresc., 1996, vol. 6, no. 3, p. 147.

    Article  Google Scholar 

  5. Le Guern, F., Mussard, V., Gaucher, A., et al., Int. J. Mol. Sci., 2020, vol. 21, no. 23, 9217.

    Article  Google Scholar 

  6. Gerasimova, M.A., Tomilin, F.N., Malyar, E.Yu., et al., Dyes Pigm., 2020, vol. 173, 107851.

    Article  Google Scholar 

  7. Alvarez-Pez, J.M., Ballesteros, L., Talavera, E., et al., J. Phys. Chem. A, 2001, vol. 105, p. 6320.

    Article  Google Scholar 

  8. Surzhikova, D., Gerasimova, M., Tretyakova, V., et al., J. Photochem. Photobiol., A, 2021, vol. 413, 113233.

    Article  Google Scholar 

  9. Yguerabide, J., Talavera, E., Alvarez, J.M., et al., Photochem. Photobiol., 1994, vol. 60, p. 435.

    Article  Google Scholar 

  10. Slyusareva, E.A. and Gerasimova, M.A., Russ. Phys. J., 2014, vol. 56, no. 12, p. 1370.

    Article  Google Scholar 

  11. Surzhikova, D.P., Gerasimova, M.A., and Slyusareva, E.A., Russ. Phys. J., 2022, vol. 64, no. 11, p. 2102.

    Article  Google Scholar 

  12. Mchedlov-Petrosyan, N.O., Vestn. Kharkov. Nats. Univ., 2004, vol. 626, no. 11(34), p. 221.

Download references

ACKNOWLEDGMENTS

The authors are grateful to A. Plotnikov for his help in analyzing our experimental data.

Funding

This work was supported by the Russian Science Foundation, project no. 22-22-00724.

Author information

Authors and Affiliations

  1. Siberian Federal University, 660041, Krasnoyarsk, Russia

    D. S. Surzhikova, M. A. Gerasimova & E. A. Slyusareva

Authors
  1. D. S. Surzhikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Gerasimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Slyusareva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. S. Surzhikova.

Ethics declarations

The authors declare that they have no conflicts of interest.

Additional information

Translated by E. Bondareva

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Surzhikova, D.S., Gerasimova, M.A. & Slyusareva, E.A. Effect of Phosphate Ions on the Dianion–Anion Equilibrium of Fluorescein Excited State. Bull. Russ. Acad. Sci. Phys. 86, 1203–1206 (2022). https://doi.org/10.3103/S1062873822100215

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S1062873822100215

Search

Navigation