Optical Analysis of Implants from the Dura Mater
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Presents the results of the spectral analysis using the method of Raman scattering spectroscopy (RS) of dura mater (DM) samples, manufactured by technology “Lioplast” practised in the clinic in the area of atrophic processes at multiple gum recessions. The method of Fourier deconvolution and selection of the spectral profile by the method of least squares is used to increase the resolution and informativity of the spectrum. With the help of mathematical methods of separation of overlapping spectral contours, the main bands corresponding to the main components of the implants were found: amides, proteins, glycosaminoglycans, DNA/RNA. On the basis of the two-dimensional spectral analysis, the coefficients reflecting the composition of the dura mater with different methods of its treatment were introduced. It has been established that Raman spectroscopy can be used to evaluate implants from the dura mater.
KeywordsRaman spectroscopy dura mater biomaterial spectral analysis Fourier deconvolution modeling of the spectral contour
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- 1.Muslimov, S.A., Morfologicheskie aspekty regenerativnoy khirurgii (Morphological Aspects of Regenerative Surgery), Ufa: Bashkortostan, 2000.Google Scholar
- 2.Ganja, I.R., Recession of the Gums, in Diagnostics and Methods of Treatment: A Manual for Doctors, Ganja, I.R., Modina, T.N., and Khamadeeva, A.M., Eds., Samara: Commonwealth, 2007.Google Scholar
- 3.Ferraro, J.R. and Nakamoto, K., Introductory Raman Spectroscopy, San Diego: Academic, 1994.Google Scholar
- 6.Saxena, T., Deng, B., Stelzner, D., Hasenwinkel, J., and Chaiken, J., Raman spectroscopic investigation of spinal cord injury in a rat model, J. Biomed. Opt., 2011, vol. 16, no. 2, Article 027003.Google Scholar
- 7.Bazarnova, N.G., Karpova, E.V., Katrakov, I.B., et al., Methods of Studying Wood and Its Derivatives. Tutorial Text, Barnaul: Izd-vo Altai Gos. Univ., 2002 [in Russian].Google Scholar
- 10.Timchenko, E.V., Timchenko, P.E., Volova, L.T., Dolgushkin, D.A., Shalkovsky, P.Y., and Pershutkina, S.V., Detailed spectral analysis of decellularized skin implants, J. Phys.: Conf. Ser., 2016, vol. 737, no. 012050, pp. 1–4.Google Scholar
- 11.Timchenko, P.E., Timchenko, E.V., Pisareva, E.V., Vlasov, M.Yu., Red’kin, N.A., and Frolov, O.O., Spectral analysis of allogeneic hydroxyapatite powders, J. Phys.: Conf. Ser., 2017, vol. 784, p. 012060. doi 10.1088/1742-6596/784/1/012060Google Scholar
- 12.Thomas, G.J., Jr., Raman spectroscopy of viruses and protein-nucleic acid interactions, SPEX Speacker, 1976, vol. XXI, no.4.Google Scholar
- 16.Ruiz-Chica, A.J., Medina, M.A., Sanchez-Jimenez, F., and Ramirez, F.J., Characterization by Raman spectroscopy of conformational changes on guaninecytosine and adenine-thymine oligonucleotides induced by aminooxy analogues of spermidine, J. Raman Spectrosc., 2004, vol. 35, pp. 93–100.CrossRefGoogle Scholar
- 18.Rudd, T.R.1, Hussain, R., Siligardi, G., and Yates, E.A., Raman and Raman optical activity of glycosaminoglycans, 2010, vol. 46, no. 23, pp. 4124–4126. doi 10.1039/c001535kGoogle Scholar
- 19.Motulsky, H.J. and Christopoulos, A., Fitting models to biological data using linear and nonlinear regression, in A Practical Guide to Curve Fitting, San Diego, CA: GraphPad Software Inc., 2003.Google Scholar