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MALDI-TOF Mass Spectrometry Identification of the Marker of Vibrio cholerae Toxicity

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Abstract

This study was aimed at MALDI-TOF identification of protein markers of V. cholerae toxicity for express diagnostics of the causative agent of cholera. The MALTDI-TOF MS electronic passports of 140 V. cholerae strains that were divided into two main groups with the presence or absence of the gene of cholera toxin (ctx– and ctx+) were subjected to computer analysis. For the first time, we have identified a taxon, a specific 3202 Da protein peak, that is a characteristic marker of V. cholerae ctx+, but not of V. cholerae ctx– strains.

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Authors and Affiliations

  1. Department of General and Clinical Biochemistry No. 1, Rostov State Medical University, 344022, Rostov-on-Don, Russia

    S. O. Chaika, N. R. Telesmanich & Yu. M. Lomov

Authors
  1. S. O. Chaika
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  2. N. R. Telesmanich
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  3. Yu. M. Lomov
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Correspondence to S. O. Chaika.

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Translated by E. Berezhnaya

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Chaika, S.O., Telesmanich, N.R. & Lomov, Y.M. MALDI-TOF Mass Spectrometry Identification of the Marker of Vibrio cholerae Toxicity. Mol. Genet. Microbiol. Virol. 33, 145–146 (2018). https://doi.org/10.3103/S0891416818020052

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  • DOI: https://doi.org/10.3103/S0891416818020052

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