Frequency of Canine Parvovirus Type-2 Variants by PCR in Enteric and Healthy Dogs and Genotyping of Variants by Restriction Enzyme (RE) Mapping by DdeI Endonuclease Based on Partial VP-2 Gene Sequence
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Canine parvovirus 2 (CPV-2) is the causative agent of myocarditis and acute hemorrhagic enteritis in dog populations. In this study, vaccinal strains and sixty wild strains (CPV-2a, CPV-2b) of CPV-2 from fecal samples of enteric (30 strains) and healthy (30 strains) dogs detected by Duplex-PCR, were compared together by application of RE mapping based on a partial sequence of VP2 gene (681 bp) by DdeI endonuclease. Our results showed that both CPV-2a and CPV-2b are pathogenic for dogs, but CPV-2a are more prevalent in healthy dogs while CPV-2b strains are more prevalent in enteric cases, significantly (p < 0.05). RE mapping based on DdeI restriction endonuclease demonstrated homology in the nucleotide sequence in amplified part of VP2 gene region in the genome of vaccinal and wild strains of CPV-2 detected in fecal samples of tested animals.
Keywords:canine parvovirus-2 VP2 genotyping restriction enzyme mapping DdeI
Authors express their willing’s to the Head of Faculty of Veterinary Medicine, Semnan University for providing facilities for the present study and E. Atefi Tabar and Behnaz Raeisian for their cooperation’s in laboratory experiments.
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