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The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen

Molecular Genetics, Microbiology and Virology volume 32, pages 100–108 (2017)Cite this article

Abstract

The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the B. cereus group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment.

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Affiliations

  1. State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279, Russia

    I. Yu. Shchit, T. Yu. Kudryavtseva, N. A. Shishkova, R. I. Mironova, L. I. Marinin, A. N. Mokrievich, S. F. Biketov & I. A. Dyatlov

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971, Russia

    K. B. Ignatov & V. M. Kramarov

  3. All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, 127422, Russia

    K. B. Ignatov & V. M. Kramarov

Authors
  1. I. Yu. Shchit
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  2. K. B. Ignatov
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  3. T. Yu. Kudryavtseva
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  4. N. A. Shishkova
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  5. R. I. Mironova
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  6. L. I. Marinin
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  7. A. N. Mokrievich
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  8. V. M. Kramarov
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  9. S. F. Biketov
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  10. I. A. Dyatlov
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Corresponding author

Correspondence to A. N. Mokrievich.

Additional information

Original Russian Text © I.Yu. Shchit, K.B. Ignatov, T.Yu. Kudryavtseva, N.A. Shishkova, R.I. Mironova, L.I. Marinin, A.N. Mokrievich, V.M. Kramarov, S.F. Biketov, I.A. Dyatlov, 2017, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2017, No. 2, pp. 69–76.

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Shchit, I.Y., Ignatov, K.B., Kudryavtseva, T.Y. et al. The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen. Mol. Genet. Microbiol. Virol. 32, 100–108 (2017). https://doi.org/10.3103/S0891416817020094

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  • DOI: https://doi.org/10.3103/S0891416817020094

Keywords

  • loop isothermal amplification of DNA
  • LAMP
  • Bacillus anthracis