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A retrospective macrorestrictive analysis of Vibrio cholerae eltor strains isolated under epidemic complications in the Russian Far East

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Abstract

A retrospective study of the clonal structure of 24 Vibrio cholerae eltor strains isolated during epidemic complications in the Russian Far East was carried out on the basis of a macrorestrictive analysis of Vibrio cholerae eltor genomic DNA using NotI and SfiI endonucleases. It was found that clonal (by amplification profiling data) toxigenic strains (n = 23) were characterized by variability of the NotI/SfiI-generated restriction profiles. The V. cholerae eltor isolated during an outbreak in the city of Yuzhno-Sakhalinsk were differentiated into four pulse types, with one dominating type detected in 76.5% of the strains. A separate NotI/SfiI-restriction profile was characteristic for the isolates from Primorskii krai. A nontoxigenic strain formed a separate dendrogram line from the toxigenic strains on the basis of the PFGE-pattern structure. The use of macrorestriction analysis was shown to be effective for deep characterization of the V. cholerae population structure and for ascertainment of molecular-epidemiological regularities in the territorial distribution of certain V. cholerae clones.

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References

  1. Laboratornaya diagnostika kholery: Metodicheskie ukazaniya MUK 4.2.2218-07 (Molecular Diagnostics of Cholera: Guidelines MUK 4.2.2218-07), Moscow: Fed. Tsentr Gig. Epidemiol., 2007.

  2. Maramovich, A.S., Urbanovich, L.Ya., Mironova, L.V., and Kulikalova, E.S., J. Microbiol. Epidemiol. Immunobiol., 2006, vol. 6, pp. 63–71.

    Google Scholar 

  3. Medinskii, G.M. and Lomov, Yu.M., Kholera v SSSR v period sed’moi pandemii (Cholera in the USSR during the Seventh Pandemia), Pokrovskii, V.I., Ed., Moscow, 2000.

  4. Mironova, L.V., Balakhonov, S.V., Urbanovich, L.Ya., et al., Mol. Genet., 2012, vol. 2, pp. 13–20.

    Google Scholar 

  5. Onishchenko, G.G., Maramovich, A.S., Golubinskii, E.P., et al., J. Microbiol. Epidemiol. Immunobiol., 2000, vol. 5, pp. 26–31.

    Google Scholar 

  6. Onishchenko, G.G., Maramovich, A.S., Golubinskii, E.P., et al., J. Microbiol. Epidemiol. Immunobiol., 2000, vol. 5, pp. 31–35.

    Google Scholar 

  7. Barrett, T.J., Gerner-Smidt, P., and Swaminathan, B., Foodborne Pathog. Dis., 2, vol. 3, no. 1, pp. 20–31.

  8. Chin, C.S., Sorenson, J., Harris, J.B., et al., N. Engl. J. Med., 2011, vol. 364, pp. 33–42.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Cooper, K.L., Luey, C.K., Bird, M., et al., Foodborne Pathog. Dis., 2006, vol. 3, no. 1, pp. 51–58.

    Article  CAS  PubMed  Google Scholar 

  10. Foley, S.L., Lynne, A.M., and Nayak, R., Infect. Genet. Evol., 2009, vol. 9, no. 4, pp. 430–440.

    Article  CAS  PubMed  Google Scholar 

  11. Gilmour, M.W., Martel-Laferriere, V., Lévesque, S., et al., Emerg. Infect. Dis., 2011, vol. 17, no. 6, pp. 1124–1125.

    Article  PubMed Central  PubMed  Google Scholar 

  12. Hunter, P.R. and Gaston, M.A.J., Clin. Microbiol, 1988, vol. 26, no. 11, pp. 2465–2466.

    CAS  Google Scholar 

  13. Kam, K.M., Luey, C.K., Tsang, Y.M., et al., J. Clin. Microbiol., 2003, vol. 41, no. 10, pp. 4502–4511.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Keddy, K.H., Nadan, S., Govind, C., and Sturm, A.W., J. Med. Arch. Microbiol., 2007, vol. 56, no. 12, pp. 1644–1650.

    Article  CAS  Google Scholar 

  15. Morbidity and Mortality Weekly Report, 2010, vol. 59, no. 45, pp. 174–179.

    Google Scholar 

  16. Morita, M., Ohnishi, M., Arakawa, E., et al., Microbiol. Immunol., 2008, vol. 52, pp. 314–317.

    Article  CAS  PubMed  Google Scholar 

  17. Nusrin, S., Khan, G.Y., Bhuiyan, N.A., et al., J. Clin. Microbiol., 2004, vol. 42, no. 12, pp. 5854–5856.

    Article  PubMed Central  PubMed  Google Scholar 

  18. Rapid standard laboratory protocol for molecular subtyping of Vibrio cholerae by pulse-field gel electrophoresis (PFGE), in The National Molecular Subtyping Network for Foodborne Disease Surveillance, Pulsenet, USA: Centers Dis. Control Prevent., 2006.

  19. Talkington, D., Boop, C., Tarr, C., et al., Emerg. Infect. Dis., 2011, vol. 17, no. 11, pp. 2122–2129.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Tenover, F.C., Arbeit, R.D., Goering, R.V., et al., J. Clin. Microbiol., 1995, vol. 33, no. 9, pp. 2233–2239.

    CAS  PubMed Central  PubMed  Google Scholar 

  21. The National Molecular Subtyping Network for Foodborne Disease Surveillance. http://www.pulsenetinter-national.org. Cited December 15, 2012.

  22. van Belkum, A., Tassios, P.T., Dijkshoorn, L., et al., J. Clin. Microbiol. Infect., 2007, vol. 13, suppl. 3, pp. 1–46.

    Article  Google Scholar 

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Authors and Affiliations

  1. Irkutsk Antiplague Research Institute, Federal Service for Supervision of Consumer Rights Protection and Human Welfare, ul. Trilissera 78, Irkutsk, 664047, Russia

    L. V. Mironova, M. V. Afanasev, E. A. Basov, L. Ya. Urbanovich & S. V. Balakhonov

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  1. L. V. Mironova
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  2. M. V. Afanasev
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  3. E. A. Basov
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  4. L. Ya. Urbanovich
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  5. S. V. Balakhonov
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Correspondence to L. V. Mironova.

Additional information

Original Russian Text © L.V. Mironova, M.V. Afanasev, E.A. Basov, L.Ya. Urbanovich, S.V. Balakhonov, 2014, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2014, No. 2, pp. 23–36.

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Mironova, L.V., Afanasev, M.V., Basov, E.A. et al. A retrospective macrorestrictive analysis of Vibrio cholerae eltor strains isolated under epidemic complications in the Russian Far East. Mol. Genet. Microbiol. Virol. 29, 77–85 (2014). https://doi.org/10.3103/S0891416814020074

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  • DOI: https://doi.org/10.3103/S0891416814020074

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