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Molecular typing of Francisella tularensis using multiple-locus variable-number tandem-repeat analysis

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Abstract

Variable-number tandem-repeat analysis (VNTR) of 25 loci was used for molecular typing of the Francisella tularensis strains isolated from different regions of Russia and the former Soviet Union. This approach allowed us to subdivide F. tularensis subspecies and determine genotype diversity with regard to geographical prevalence. All 25 loci were examined for their ability to distinguish subspecies and local geographical groups. Forty-two genotypes of the 58 investigated F. tularensis subsp. holarctica isolates were found using cluster VNTR analysis.

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Authors and Affiliations

  1. State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279, Russia

    V. S. Timofeev, T. Yu. Kudryavtseva, A. N. Mokrievich, V. M. Pavlov & I. A. Dyatlov

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  1. V. S. Timofeev
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  2. T. Yu. Kudryavtseva
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  3. A. N. Mokrievich
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  4. V. M. Pavlov
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  5. I. A. Dyatlov
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Corresponding author

Correspondence to A. N. Mokrievich.

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Original Russian Text © V.S. Timofeev, T.Yu. Kudryavtseva, A.N. Mokrievich, V.M. Pavlov, I.A. Dyatlov, 2014, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2014, No. 1, pp. 8–15.

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Timofeev, V.S., Kudryavtseva, T.Y., Mokrievich, A.N. et al. Molecular typing of Francisella tularensis using multiple-locus variable-number tandem-repeat analysis. Mol. Genet. Microbiol. Virol. 29, 8–15 (2014). https://doi.org/10.3103/S0891416814010066

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  • DOI: https://doi.org/10.3103/S0891416814010066

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