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Biofilms of Burkholderia cepacia: Characteristics of mutants with modified formation capacity

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Abstract

The bacteria Burkholderia cenocepacia, which cause pulmonary infections in people with low immunity and in children with inherited diseases such as cystic fibrosis, were studied. The virulence of these bacteria is related with their ability to form viscous biofilms in the lung bronchi and alveoli of patients. Data on the possibility of producing mutants with an altered changed biofilm formation ability using insertion mutagenesis and their characteristics in vivo and in vitro are presented. Three genes involved in the genetic regulation of biofilm formation were identified by cloning and sequencing fragments of mutant genes. Two of the identified genes are the global regulatory genes ompR and tetR, and their mutations result in a decrease or higher ability to form biofilms of B. cenocepacia. Proteomic analysis identified the proteins in mutant strains with a different expression level than in the initial strains. The adhesive properties of the biofilms formed by initial and mutant strains of Burkholderia were evaluated using light and scanning microscopy in an analysis of the interaction between these strains and abiotic surfaces and cell cultures of pulmonary epithelium HL. It was shown that mutant strains with a low ability to form biofilms were characterized by a disturbed adhesion process.

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References

  1. Avtandilov, G.G., Nevzorov, V.P., and Nevzorova, O.F., Sistemnyi stereometricheskii analiz ul’trastruktur kletok (Systemic Stereometric Analysis of Cellular Ultrastructures), Chisinau, 1984.

  2. Zaitseva, Yu.V., Voloshina, P.V., and Liu, X., Russ. J. Genet., 2010, vol. 46, no. 5, pp. 616–621.

    Article  Google Scholar 

  3. Il’ina, T.S, Romanova, Yu.M., and Gintsburg, A.L., Russ. J. Genet., 2004, vol. 40, no. 11, pp. 1–12.

    Google Scholar 

  4. Maniatis, T., Fritsch, E. F., and Sambrook, J. Molecular Cloning, Cold Spring Harbor, New York: Cold Spring Harbor Lab. Press, 1982. Translated under the title Metody geneticheskoi inzhenerii. Molekulyarnoe klonirovanie Moscow: Mir, 1984.

    Google Scholar 

  5. Romanova, Yu.M., Alekseeva, N.V., Smirnova, T.A., et al., Zh. Mikrobiol. Epidemiol. Immunobiol., 2006, no. 4, pp. 38–42.

  6. Romanova, Yu.M., Stepanova, T.V., Nestervnko, L.N., et al., Zh. Mikrobiol. Epidemiol. Immunobiol., 2009, no. 4, pp. 29–33.

  7. Smirnova, T.A., Didenko, L.V., Tiganova, I.G., et al., Biotekhnologiya, 2009, no. 5, pp. 16–23.

  8. Smirnova, T.A., Didenko, L.V., Azizbekyan, R.R., and Romanova, Yu.M., Microbiology, 2010, vol. 79, no. 4, pp. 435–446.

    Article  Google Scholar 

  9. Tiganova, I.G., Romanova, Yu.M., Galkina, S.I., et al., Epid. Sanit., 2009, no. 15, pp. 22–26.

  10. Shaginyan, I.A., Khmel’, I.A., Romanova, Yu.M., et al., Mol. Genet., 2003, no. 4, pp. 15–20.

  11. Costerton, J.W. and Irvin, R., T., Cheng K.J, Crit. Rev. Microbiol., 1981, vol. 8, pp. 303–338.

    Article  PubMed  CAS  Google Scholar 

  12. Costerton, J.W., Stewart, P.S., and Greenberg, E.P., Science, 1999, vol. 284, pp. 318–322.

    Article  Google Scholar 

  13. Cunha, M.V., Sousa, S.A., and Leitao, J.H., et al., J. Clin. Microbiol., 2004, vol. 42, pp. 3052–3058.

    Article  PubMed  CAS  Google Scholar 

  14. Dennis, J.J. and Zylstra, G.J., Appl. Environ. Microbiol., 1998, vol. 64, pp. 2710–2715.

    PubMed  CAS  Google Scholar 

  15. Ensgraber, M. and Loss, M., Infect. Immun., 1992, vol. 60, pp. 3072–3078.

    PubMed  CAS  Google Scholar 

  16. Ito, S. and Karnovsky, M., J. Cell Biol., 1969, vol. 39, pp. 168–169.

    Google Scholar 

  17. Hennequin, C., Porchtray, F., Waligora-Dupriet, A., et al., Microbiology, 2001, vol. 147, pp. 87–96.

    PubMed  CAS  Google Scholar 

  18. Hu, Y., Wang, Y., Ding, L., et al., Microbiology, 2009, vol. 155, pp. 3622–3631.

    Article  PubMed  CAS  Google Scholar 

  19. Karatan, E. and Watnick, P., Microbiol. Mol. Biol. Rev., 2009, vol. 73, pp. 310–347.

    Article  PubMed  CAS  Google Scholar 

  20. LiPurna, J.J., Spilker, T., Coenye, T., and Gonzales, C.F., Lancet, 2002, vol. 359, p. 2002.

    Article  Google Scholar 

  21. Loisel-Meyer, S. de Baques, M.P.J., et al., J. Bacteriol., 2005, vol. 187, pp. 2715–2726.

    Article  Google Scholar 

  22. Loutet, S.A. and Valvano, M.A., Infect. Immun., 2010, vol. 78, pp. 4088–4100.

    Article  PubMed  CAS  Google Scholar 

  23. Ramos, J.L., Marti’nez-Bueno, M., Molina-Herares, A.J., et al., Microbiol. Mol. Biol. Rev., 2005, vol. 62, pp. 326–356.

    Article  Google Scholar 

  24. O’Toole, G.A. and Kolter, R., Mol. Microbiol., 1998, vol. 30, pp. 295–304.

    Article  PubMed  Google Scholar 

  25. Vidal, O., Longin, R., Pringent-Combaret, C., et al., J. Bacteriol., 1998, vol. 180, pp. 2442–2449.

    PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation, Moscow, Russia

    Yu. M. Romanova, I. G. Tiganova, N. V. Alekseeva, T. V. Stepanova, N. V. Shevlyagina, L. V. Didenko & A. L. Ginzburg

  2. Moscow State University, Moscow, Russia

    S. N. Goryachev

  3. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia

    I. A. Khmel’

Authors
  1. Yu. M. Romanova
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  2. I. G. Tiganova
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  3. I. A. Khmel’
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  4. N. V. Alekseeva
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  5. T. V. Stepanova
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  6. N. V. Shevlyagina
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  7. L. V. Didenko
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  8. S. N. Goryachev
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  9. A. L. Ginzburg
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Additional information

Original Russian Text © Yu.M. Romanova, I.G. Tiganova, I.A. Khmel’, N.V. Alekseeva, T.V. Stepanova, N.V. Shevlyagina, L.V. Didenko, S.N. Goryachev, A.L. Ginzburg, 2011, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2011, No. 3, pp. 3–10.

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Romanova, Y.M., Tiganova, I.G., Khmel’, I.A. et al. Biofilms of Burkholderia cepacia: Characteristics of mutants with modified formation capacity. Mol. Genet. Microbiol. Virol. 26, 93–101 (2011). https://doi.org/10.3103/S0891416811030037

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  • DOI: https://doi.org/10.3103/S0891416811030037

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