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Incidence of genotype of hepatitis B subvirus and HBsAg subtypes in native people of northern and southeastern Siberia

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Abstract

Serological markers and DNA of hepatitis B virus (HBV) were detected in 487 blood samples of aboriginal people in the Alar district of the Irkutsk region (mostly Buryat) in 2005. HBsAg was found in 40 (8.2%) samples. HBV DNA was found in 24 out of 40 (60%) HBsAg-positive samples. HBV-positive DNA samples were found to contain nucleotide sequences of the Pre-S1, Pre-S2, and S regions of the HBV genome with a total length of 1146 n. 22 out of 24 (92%) isolates were found to belong to the D genotype, two belonged to the C genotype; eight (33.3%) belonged to the D3 subgenotype, six (25.0%) belonged to the D2 subgenotype, one (4.1%) belonged to the D1 subgenotype, and nine (37.5%) belonged to unidentified subgenotype. The incidence of the HBsAg subtype was determined to be ayw2 in 14 out of 24 (58.3%) isolates and ayw3 in seven (29.2%) isolates; the subtype was not identified for one (4.1%) isolate. In two C-genotype isolates, the subtypes were identified as adw2 and adrq+. A comparative analysis of the results of this work and those obtained previously for native people of Yamalo-Nenets Autonomous District (YaNAD, mostly Khanty and Komi) demonstrated significant differences in the incidence in YaNAD of HBsAg (3.2% isolates, p > 0.999), subgenotype D2 (62% isolates, p > 0.95), and subtype ayw3 (70.6% isolates; p > 0.95). The variability in the incidence of two variants in two groups of native Siberian peoples is the evidence of different infection sources in these populations.

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Authors and Affiliations

  1. Helicon, Ltd., 119992, Moscow, Russia

    V. A. Manuilov

  2. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    L. P. Osipova

  3. Vector-Best, Koltsovo, Novosibirsk oblast, Russia

    I. G. Netesova & L. V. Tsoy

  4. Vector State Research Center of Virology and Biotechnology, Rospotrebnadzor, Koltsovo, Novosibirsk oblast, Russia

    E. V. Chub & S. V. Netesov

  5. Alar Central Regional Hospital, Kutulik, Irkutsk oblast, Russia

    R. V. Dul’beev & L. R. Alekseeva

  6. Swedish Institute for Infectious Disease Control, Stockholm, Sweden

    H. Norder & L. O. Magnius

  7. Novosibirsk State University, Novosibirsk, Russia

    S. V. Netesov

Authors
  1. V. A. Manuilov
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  2. L. P. Osipova
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  3. I. G. Netesova
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  4. E. V. Chub
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  5. L. V. Tsoy
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  6. R. V. Dul’beev
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  7. L. R. Alekseeva
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  8. H. Norder
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  9. L. O. Magnius
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  10. S. V. Netesov
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Additional information

Original Russian Text © V.A. Manuilov, L.P. Osipova, I.G. Netesova, E.V. Chub, L.V. Tsoy, R.V. Dul’beev, L.R. Alekseeva, H. Norder, L.O. Magnius, S.V. Netesov, 2010, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2010, No. 4, pp. 32–37.

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Manuilov, V.A., Osipova, L.P., Netesova, I.G. et al. Incidence of genotype of hepatitis B subvirus and HBsAg subtypes in native people of northern and southeastern Siberia. Mol. Genet. Microbiol. Virol. 25, 172–177 (2010). https://doi.org/10.3103/S0891416810040063

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  • DOI: https://doi.org/10.3103/S0891416810040063

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