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Molecular genetic monitoring of Yersinia pseudotuberculosis using PCR O-genotyping

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Abstract

PCR O-genotyping of 117 strains of Y. pseudotuberculosis, serotype O:1-O:4, isolated in Siberia and in the Far East has been performed. This method allows identification of both O-genotype and its variants (a, b, c). It has been shown that three O-genotypes of Y. pseudotuberculosis (O:1a, O:1b, and O:3) circulate in Siberia and six types (O:1a, O:1b, O:1c, O:2a, O:3, and O:4b) circulate in the Far East. Genotype O:1b dominates in both regions (87.8%). A PCR algorithm for identification of epidemically significant Y. pseudotuberculosis O-genotypes has been developed. The PCR analysis of clinical material makes it possible to identify the O-genetic type of an etiological agent without bacteriological isolation of a pathogen.

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Authors and Affiliations

  1. Antiplague Research Institute of Siberia and the Far East, ul. Trilissera 78, Irkutsk, 664047, Russia

    V. T. Klimov & M. V. Chesnokova

Authors
  1. V. T. Klimov
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  2. M. V. Chesnokova
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Original Russian Text © V.T. Klimov, M.V. Chesnokova, 2007, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2007, No. 4, pp. 14–16.

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Klimov, V.T., Chesnokova, M.V. Molecular genetic monitoring of Yersinia pseudotuberculosis using PCR O-genotyping. Mol. Genet. Microbiol. Virol. 22, 150–154 (2007). https://doi.org/10.3103/S0891416807040039

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  • DOI: https://doi.org/10.3103/S0891416807040039

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