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Bacterial Complexes in Fens of Tomsk Oblast: Abundance, Taxonomic Structure, an Activity

  • SOIL BIOLOGY
  • Published:
Moscow University Soil Science Bulletin Aims and scope

Abstract

This article contains an analysis of structural and functional parameters of bacterial complexes taken for examination from complete profiles of fens assigned to the Klyukvennoe and Karbyshevskoe wetlands in Tomsk oblast. Bacteria in the fens are revealed throughout the entire profile. Their number determined by a direct method of fluorescence microscopy is high and varies from 27 to 78 × 109 cells/g of dry peat. The high number of saprotrophic bacteria (to 108–109 CFU/g of dry peat) is detected by the plate method. An increase in the number of saprotrophic bacteria down the fen profile is revealed. These data enable us to conclude for the first time that index k (a dimensionless universal characteristic of natural prokaryotic communities) in fen profiles decreases with depth. The bacterial cultures isolated from the fens studied are assigned to ten taxa: Bacillus, Streptomyces, Arthrobacter, Microbacterium, Pseudomonas, Flavobacterium, Dyadobacter, Janthinobacterium, Pedobacter, and Myxobacteriales. The acrotelm is dominated by bacterial cultures represented by genera Bacillus and Streptomyces; and Pseudomonas, Dyadobacter, and Arthrobacter are the dominants of the catotelm. The functions of bacterial genera and species identified by molecular biological methods are described. The study of nitrogen fixation, denitrification, and methanogenesis has shown that bacteria are viable and active throughout the entire peat profile.

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Funding

This work was supported by the Russian Foundation for Basic Research and was performed according to the scientific, project no. 19-29-05197.

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Authors and Affiliations

  1. Department of Soil Science, Moscow State University, Moscow, Russia

    A. V. Golovchenko, Ju. D. Dmitrienko, T. G. Dobrovolskaya, T. A. Gracheva & P. A. Kozhevin

  2. Tomsk State Pedagogical University, Tomsk, Russia

    L. I. Inisheva

Authors
  1. A. V. Golovchenko
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  2. Ju. D. Dmitrienko
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  3. T. G. Dobrovolskaya
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  4. T. A. Gracheva
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  5. L. I. Inisheva
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  6. P. A. Kozhevin
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Correspondence to A. V. Golovchenko.

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Translated by I. Bel’chenko

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Golovchenko, A.V., Dmitrienko, J.D., Dobrovolskaya, T.G. et al. Bacterial Complexes in Fens of Tomsk Oblast: Abundance, Taxonomic Structure, an Activity. Moscow Univ. Soil Sci. Bull. 75, 184–191 (2020). https://doi.org/10.3103/S0147687420040031

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  • DOI: https://doi.org/10.3103/S0147687420040031

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