Abstract
From the root surface of vegetable plants growing in the soils of North Vietnam, 227 pure bacterial cultures are isolated on nitrogen-free media. All the cultures show nitrogen-fixing ability and positive effect on plant growth in model experiments, while none of them cause the formation of root nodules or tumors. Based on the gene sequencing of the 16S rRNA, our determination of the phylogenic position of the most active isolated strains reveals their affiliation with the family Rhizobiaceae of the phylum Alpharoteobacteria and that they are closest to the type strain of the genus Agrobacterium—A. tumefaciens. The physiology and morphological features also indicate the affiliation of these organisms with A. tumefaciens. Thus, our isolated strain of this plant pathogen bacterium appears as associative diazotrophs, which also have a positive effect on the growth of cultural plants.
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References
Grodzinskii, A.M. and Grodzinskii, D.M., Kratkii spravochnik po fiziologii rastenii (Brief Handbook on the Plant Physiology), Kyiv: Naukova Dumka, 1964.
Emtsev, V.T., Associated symbiosis of soil diazotrophic bacteria and vegetables, Pochvovedenie, 1995, no. 4, pp. 74–78.
Zavalin, A.A., Chistotin, M.F., and Kozhemyakov, A.P., Efficiency of inoculation of cereals with Agrobacterium radiobacter depending on nitrogen-containing fertilizers, soil and meteorological conditions, Agrokhimiya, 2001, no. 2, pp. 31–3.
Zvyagintsev, D.G., Metody pochvennoi mikrobiologii (Practical Manual on Soil Microbiology), Moscow: Mosk. Gos. Univ., 1991.
Manucharova, N.A., Gidroliticheskie prokariotnye kompleksy nazemnykh ekosistem (Hydrolytic Prokaryotic Complexes in Terrestrial Ecosystems), Moscow: Universitetskaya Kniga, 2014.
Manucharova, N.A., Vlasenko, A.N., Stepanov, A.L., Zenova, G.M., Tourova, T.P., and Panteleeva, A.N., Thermophilic chitinolytic microorganisms of brown semidesert soil, Microbiology (Moscow), 2008, vol. 77, no. 5, pp. 5
Manual of Methods for General Bacteriology, Gerhardt, P., Ed., Washington D.C.: Am. Soc. Microbiol., 1981, vol. 3.
Netrusov, A.I., Egorova, M.A., Zakharchuk, L.M., et al., Praktikum po mikrobiologii (Practical Manual on Microbiology), Moscow: Akademiya, 2005.
Tikhonovich, I.A. and Provorov, N.A., Simbiozy rastenii i mikroorganizmov: molekulyarnaya genetika agrosistem budushchego (Symbiosis of the Plants and Microorganisms: Molecular Genetics of Prospective Agrosystems), St. Petersburg: S.-Peterb. Gos. Univ., 2009.
Bergey’s Manual of Determinative Bacteriology, Holt, J.G., Krieg, N.R. Sneath, P.H.A., Staley, J.T., and Williams, S.T., Eds., Baltimore, MD: Williams & Wilkins, 1994, 9th ed.
El-Fiki, F. and Giles, K.L., Agrobacterium tunerfaciens in agriculture and research, Int. Rev. Cytol., 1981, vol. 13, suppl., pp. 47–58.
Heumann, W., Rhizobium genetics, in Biology of Inorganic Nitrogen and Sulphur, Bothe, H. and Trebst, A., Eds., New York, 1981, pp. 87–102.
Jordan, D.C., Rhizobiaceae, in Bergey’s Manual of Determinative Bacteriology, Holt, J.G., and Krieg, N.R., Eds., Baltimore, MD: Williams & Wilkins, 1984, pp. 234–256.
Kanvinde, L. and Sastry, G.R.K., Agrobacterium tumefaciens is a diazotrophic bacterium, Appl. Environ. Microbial., 1990, vol. 56, no. 7, pp. 2087–2092.
The Rhizobiaceae, Spaink, H., Kondorosi, A., and Hooykaas, P.J.J., Eds., Dordrecht, Netherlands: Kluwer, 1998.
Robert, G.P. and Brill, W.J., Genetics and regulation of nitrogen fixation, Ann. Rev. Microbiol., 1981, vol. 35, pp. 207–235.
Ruvkun, G.B. and Ausubel, F.M., Interspecies homology of nitrogenase, Proc. Natl. Acad. Sci. U.S.A., 1980, vol. 77, pp. 191–195.
Smith, E.F. and Townsend, C.O., A plant tumor of bacterial origin, Science, 1907, vol. 25, pp. 671–673.
Wood, D.W., Setubal, J.C., Kaul, R., et al., The genome of the natural genetic engineer Agrobacterium tumefaciens C58, Science, 2001, vol. 294, pp. 2317–2323.
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Original Russian Text © Phung Thi My, N.A. Manucharova, A.L. Stepanov, L.A. Pozdnyakov, O.V. Selitskaya, V.T. Emtsev, 2015, published in Vestnik Moskovskogo Universiteta. Pochvovedenie, 2015, No. 3, pp. 50–55.
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My, P.T., Manucharova, N.A., Stepanov, A.L. et al. Agrobacterium tumefaciens as associative nitrogen-fixing bacteria. Moscow Univ. Soil Sci. Bull. 70, 133–138 (2015). https://doi.org/10.3103/S0147687415030047
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DOI: https://doi.org/10.3103/S0147687415030047