Skip to main content
SpringerLink
Log in

Peculiarities of Cytochrome c Enzymatic Activity with Cardiolipin

  • SHORT COMMUNICATION
  • Published:
Moscow University Biological Sciences Bulletin Aims and scope Submit manuscript

Abstract

The points of enzymatic activity, quantum yields, structure, functions of luminol chemiluminescence activated by physical activators natural dyes isoquinolysine coumarins C-334 and C-525 under the action of cytochrome c complex with cardiolipin in aqueous medium and in nonpolar environment were considered. It is shown: (1) the enzymatic activity points and quantum yields are significantly higher in the presence of the physical activator coumarin C-525 than in the case of its own unactivated luminescence or in the case of the physical activator C-334; (2) the enzymatic activity depends not only on the concentration of cytochrome c but also on the percentage ratio between the native form of cytochrome c with cardiolipin and the partially denatured one.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

REFERENCES

  1. Vladimirov, Yu.A., Proskurnina, E.V., Izmailov, D.Yu., Novikov, A.A., Brusnichkin, A.V., Osipov, A.N., and Kagan, V.E., Mechanism of activation of cytochrome c peroxidase activity by cardiolipin, Biochemistry (Moscow), 2006, vol. 71, no. 9, pp. 989–997. https://doi.org/10.1134/s0006297906090070

    Article  CAS  PubMed  Google Scholar 

  2. Vladimirov, Yu.A., Proskurnina, E.V., and Izmailov, D.Yu., Chemiluminescence as a method for detection and study of free radicals in biological systems, Bull. Exp. Biol. Med., 2007, vol. 144, no. 3, pp. 390–396. https://doi.org/10.1007/s10517-007-0340-3

    Article  CAS  PubMed  Google Scholar 

  3. Vladimirov, Yu.A., Demin, E.M., Proskurnina, E.V., and Osipov, A.N., Lipoperoxide radical production during oxidation of cardiolipin in the complex with cytochrome c, Biochemistry (Moscow) Suppl. Ser. A: Membrane Cell Biol., 2009, vol. 3, no. 4, pp. 467–477. https://doi.org/10.1134/s1990747809040151

    Article  Google Scholar 

  4. Vladimirov, Yu.A., Proskurnina, E.V., Izmailov, D.Yu., Novikov, A.A., Brusnichkin, A.V., Osipov, A.N., and Kagan, V.E., Cardiolipin activates cytochrome c peroxidase activity since it facilitates H2O2 access to heme, Biochemistry (Moscow), 2006, vol. 71, no. 9, pp. 998–1005. https://doi.org/10.1134/s0006297906090082

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

Implemented within the framework of financing projects of the Limited Liability Company of the Center for Additional Professional Education “The Power of Knowledge.”

Author information

Authors and Affiliations

  1. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    I. N. Levchenko

  2. Moscow State University, 119991, Moscow, Russia

    I. N. Levchenko, I. V. Volodyaev & Y. A. Vladimirov

  3. Sechenov Moscow State Medical University, 119048, Moscow, Russia

    G. K. Vladimirov

Authors
  1. I. N. Levchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. K. Vladimirov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. V. Volodyaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. A. Vladimirov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. N. Levchenko.

Ethics declarations

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

This work does not contain any studies involving human and animal subjects.

CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

Additional information

Publisher’s Note.

Allerton Press remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Levchenko, I.N., Vladimirov, G.K., Volodyaev, I.V. et al. Peculiarities of Cytochrome c Enzymatic Activity with Cardiolipin. Moscow Univ. Biol.Sci. Bull. 78 (Suppl 1), S69–S71 (2023). https://doi.org/10.3103/S0096392523700256

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0096392523700256

Keywords:

Search

Navigation