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Cryo-Electron Microscopy at the Biological Faculty of Moscow State University

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Abstract

The paper reports an example of a successful upgrade of the JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope, which can be used to optimize sample preparation and to assess preparation quality. As a result of the instrument upgrade, it is possible to obtain subnanometer resolution of protein molecule reconstructions (within 8 Å). The role of graphene and amorphous carbon substrates in preventing the effect of preferential orientation of protein particles in a frozen sample is discussed.

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Funding

Microscope upgrading was supported by the Ministry of Science and Higher Education of the Russian Federation. Analysis of samples and obtaining three-dimensional structures of tick-borne encephalitis virus was supported by the Moscow State University Development Program (project no. 23A-Sh04-01). Development of nucleosome measurement techniques was supported by the Russian Science Foundation (project no. 19-74-30003).

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Authors and Affiliations

  1. Electron Microscopy Laboratory, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    A. V. Moiseenko

  2. Department of Chemical Enzymology, Faculty of Chemistry, Moscow State University, 119234, Moscow, Russia

    A. M. Egorov

  3. Department of Bioengineering, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    K. V. Shaitan & O. S. Sokolova

Authors
  1. A. V. Moiseenko
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  2. A. M. Egorov
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  3. K. V. Shaitan
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  4. O. S. Sokolova
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Correspondence to O. S. Sokolova.

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Translated by E. Martynova

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Moiseenko, A.V., Egorov, A.M., Shaitan, K.V. et al. Cryo-Electron Microscopy at the Biological Faculty of Moscow State University. Moscow Univ. Biol.Sci. Bull. 78 (Suppl 1), S59–S63 (2023). https://doi.org/10.3103/S0096392523700232

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