Abstract
Candida auris is a pathogenic fungus that causes infections in people with weakened immune systems. It is resistant to antimicrobial drugs, which complicates treatment. C. auris poses a serious threat to public health since it has high resistance and contagiousness. The ribosome plays an important role in the survival and development of this infection, and the search for inhibitors of the C. auris ribosome is of great importance. The authors used cryoelectron microscopy and single particle analysis to obtain the three-dimensional structure of the C. auris ribosome. Herein is described the architecture of the ribosome subunits and their interactions, providing valuable information for the development of novel antifungals against C. auris.
This is a preview of subscription content, log in via an institution to check access.
REFERENCES
Lockhart, S.R., Etienne, K.A., Vallabhaneni, S., Farooqi, J., Chowdhary, A., Govender, N.P., Colombo, A.L., Calvo, B., Cuomo, C.A., Desjardins, C.A., et al., Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses, Clin. Infect. Dis., 2017, vol. 64, no. 2, pp. 134–140. https://doi.org/10.1093/cid/ciw691
Zgadzay, Yu., Kolosova, O., Stetsenko, A., Wu, C., Bruchlen, D., Usachev, K., Validov, S., Jenner, L., Rogachev, A., Yusupova, G., Sachs, M.S., Guskov, A., and Yusupov, M., E-site drug specificity of the human pathogen Candida albicans ribosome, Sci. Adv., 2022, vol. 8, no. 21, p. 1062. https://doi.org/10.1126/sciadv.abn1062
Punjani, A., Rubinstein, J.L., Fleet, D.J., and Brubaker, M.A., cryoSPARC: Algorithms for rapid unsupervised cryo-EM structure determination, Nat. Methods, 2017, vol. 14, no. 3, pp. 290–296. https://doi.org/10.1038/nmeth.4169
Pettersen, E.F., Goddard, T.D., Huang, C.C., Couch, G.S., Greenblatt, D.M., Meng, E.C., and Ferrin, T.E., UCSF Chimera—A visualization system for exploratory research and analysis, J. Comput. Chem., 2004, vol. 25, no. 13, pp. 1605–1612. https://doi.org/10.1002/jcc.20084
Liebschner, D., Afonine, P.V., Baker, M.L., Bunkóczi, G., Chen, V.B., Croll, T.I., Hintze, B., Hung, L.-W., Jain, S., McCoy, A.J., Moriarty, N.W., Oeffner, R.D., Poon, B.K., Prisant, M.G., Read, R.J., Richardson, J.S., Richardson, D.C., Sammito, M.D., Sobolev, O.V., Stockwell, D.H., Terwilliger, T.C., Urzhumtsev, A.G., Videau, L.L., Williams, C.J., and Adams, P.D., Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix, Acta Crystallogr. Sect. D Struct. Biol., 2019, vol. 75, no. 10, pp. 861–877. https://doi.org/10.1107/s2059798319011471
Funding
The work was carried out with financial support from the Russian Science Foundation (project no. 20-64-47041).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
ETHICS APPROVAL AND CONSENT TO PARTICIPATE
This work does not contain any studies involving human and animal subjects.
CONFLICT OF INTEREST
The authors of this work declare that they have no conflicts of interest.
Additional information
Publisher’s Note.
Allerton Press remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Atamas, A.A., Guskov, A.I. & Rogachev, A.V. Structural Study of the Candida auris Ribosome. Moscow Univ. Biol.Sci. Bull. 78 (Suppl 1), S56–S58 (2023). https://doi.org/10.3103/S0096392523700220
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392523700220