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The Role of Reactive Oxygen Species in the Tone Regulation of Respiratory and Locomotor Muscle Arteries of the Rat

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Abstract—

The production of reactive oxygen species (ROS) in skeletal muscle cells increases under contraction. Respiratory and locomotor muscles differ in contraction modes; therefore, ROS production and their effect on blood vessels in these functionally different types of muscles may differ. The aim of this work was to assess the role of ROS in the tone regulation of the diaphragm artery (a. phrenica) and deep brachial artery (a. profunda brachii) of the rat. The reactions of ring preparations of the arteries were studied in the isometric regimen. The NADPH oxidase (NOX) inhibitor VAS2870 caused relaxation of the preparations; the reaction magnitude of the diaphragm arteries was greater than that of the brachial arteries: at the VAS2870 concentration of 1 μM, the contraction force decreased to 33 and 91% of the precontraction level, respectively. Tiron (\({\text{O}}_{2}^{{\centerdot - }}\) scavenger) had a similar activity profile: at the concentration of 10 mM, it caused a decrease in the contraction force of the diaphragm arteries to 38% and of the brachial arteries to 66% of the initial contraction force. Catalase, which destroys H2O2, did not affect contraction of the diaphragm arteries at the concentration of 3000 U/mL but increased contraction of the deep brachial arteries. Using the method of quantitative polymerase chain reaction, it was shown that the mRNA content of NOX isoforms, p22phox, p47phox, p67phox, Poldip2, Gpx-1, SOD-1, and catalase in the tissue of two arteries does not differ, while the content of SOD-3 mRNA in the diaphragm arteries is lower than in the brachial arteries. Thus, \({\text{O}}_{2}^{{\centerdot - }}\) produced by NOX greater increases tonic contraction of the diaphragm arteries than arteries of the locomotor muscle. H2O2, on the contrary, causes relaxation of the locomotor muscle arteries but not the diaphragm arteries. A relatively low level of SOD-3 expression may be a reason for the pronounced effect of O2– in the diaphragm arteries.

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Funding

This work was financially supported by the Russian Science Foundation, project no. 19-75-00060.

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Authors and Affiliations

  1. Institute for Biomedical Problems, Russian Academy of Sciences, 123007, Moscow, Russia

    A. A. Borzykh & O. S. Tarasova

  2. Department of Biology, Moscow State University, 119234, Moscow, Russia

    A. A. Shvetsova, I. V. Kuzmin, D. K. Gaynullina & O. S. Tarasova

  3. Department of Fundamental Medicine, Moscow State University, 119192, Moscow, Russia

    S. V. Buravkov

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  1. A. A. Borzykh
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  2. A. A. Shvetsova
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  3. I. V. Kuzmin
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  4. S. V. Buravkov
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  5. D. K. Gaynullina
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Correspondence to A. A. Borzykh.

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Statement on the welfare of animals. The experiments were carried out in compliance with the ethical standards of working with animals established by the Bioethics Commission of the Institute for Biomedical Problems of the Russian Academy of Sciences.

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Translated by D. Novikova

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Borzykh, A.A., Shvetsova, A.A., Kuzmin, I.V. et al. The Role of Reactive Oxygen Species in the Tone Regulation of Respiratory and Locomotor Muscle Arteries of the Rat. Moscow Univ. Biol.Sci. Bull. 76, 111–117 (2021). https://doi.org/10.3103/S0096392521030020

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