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Demographic Approaches to the Study of Aging on Cell Cultures

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Abstract

Aging organisms die out in accordance with the “Gompertz law,” i.e., the probability of their death increases with age. Survival curve construction is the main tool for gerontologists to study aging and test antiaging drugs. The analysis of survival curves includes obtaining some indices characterizing aging of the population, for example, the average and maximum lifespan, the mortality rate, and the aging rate. Testing geroprotectors can be correctly performed only by obtaining such curves. The dying out of stationary cell populations—bacteria, yeast, and mammalian cell cultures—also occurs in accordance with the Gompertz equation. In this regard, it is reasonable to use the construction of survival curves and their analysis to study the “aging” of non-subcultured cell cultures and testing anti-aging drugs on them. We used this approach in our experiments, due to which we were able to detect the positive anti-aging effect of the Quinton Marine Plasma on stationary phase aging culture of Chinese hamster cells.

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Funding

This study was performed under the state assignment of  Moscow State University, part 2 (basic research, no. AAAA-A16-116021660098-8).

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Authors and Affiliations

  1. Evolutionary Cytogerontology Sector, School of Biology, Moscow State University, 119234, Moscow, Russia

    A. N. Khokhlov, G. V. Morgunova & A. A. Klebanov

Authors
  1. A. N. Khokhlov
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  2. G. V. Morgunova
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  3. A. A. Klebanov
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Correspondence to A. N. Khokhlov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Khokhlov, A.N., Morgunova, G.V. & Klebanov, A.A. Demographic Approaches to the Study of Aging on Cell Cultures. Moscow Univ. Biol.Sci. Bull. 74, 262–267 (2019). https://doi.org/10.3103/S0096392519040060

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  • DOI: https://doi.org/10.3103/S0096392519040060

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