Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma

  • A. V. Orekhova
  • A. A. OsmolovskiyEmail author
  • V. G. Kreyer
  • N. A. Baranova
  • N. S. Egorov


It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X®). It was revealed that the protease of A. ochraceus, along with the RVV-X® preparation, can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus as an activator to factor X showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.


proteolytic enzymes activators of factor X diagnostics of factor X RVV-X® aspergilli protease pathological plasma. 



This work was supported by the Foundation for the Promotion of the Development of Small Enterprises in the Scientific and Technical Sphere (agreement no. 9107GU / 2015).


Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The studies were performed without the use of animals.

Statement of compliance with standards of research involving humans as subjects. The studies did not involve people as subjects.


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Copyright information

© Allerton Press, Inc. 2019

Authors and Affiliations

  • A. V. Orekhova
    • 1
  • A. A. Osmolovskiy
    • 1
    Email author
  • V. G. Kreyer
    • 1
  • N. A. Baranova
    • 1
  • N. S. Egorov
    • 2
  1. 1.Department of Microbiology, Biological Faculty, Moscow State UniversityMoscowRussia
  2. 2.International Biotechnology Center, Moscow State UniversityMoscowRussia

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