Interaction of Terbium Cations with the Donor Side of Photosystem II in Higher Plants
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Photosystem II (PSII) of higher plants provides photoinduced water oxidation and produces molecular oxygen as a by-product of this reaction followed by its release into the atmosphere. The oxygen-evolving complex (OEC) is located on the donor side of PSII and contains an Mn4CaO5 cluster catalyzing water oxidation. A cofactor of this reaction is a Ca2+ cation. Lanthanide ions are similar to Ca2+ ions in relation to some physical and chemical properties, such as the ionic radius and coordination number. In the case of calcium-binding proteins, Ca2+ substitution with these cations is possible. Some representatives of this group of cations are able to bind to the Ca-binding site of PSII. In this study, we investigated an interaction between the donor side of PSII and terbium, one of the least studied lanthanides. According to the obtained results, the incubation of native PSII preparations with Tb3+ cations caused an irreversible inhibition (by ~75% for 2 mM of Tb3+) of the oxygen evolving function. At the same time, changes in the electron transport at the acceptor side of PSII remained rather insignificant. Supplementation of an incubation buffer with 30 mM Ca2+ reduced the inhibiting effect of terbium almost two times. The obtained results agree with the fluorescence induction kinetics measurement in PSII preparations in the presence of exogenous Ca2+ and Tb3+ and allow for the supposition that terbium cations displace Ca2+ ions from OEC. This terbium-induced release of calcium from the catalytic center results in incomplete water oxidation producing H2O2 instead of molecular oxygen as it has been shown earlier for PSII in the absence of Ca2+ in OEC.
Keywords:photosystem II oxygen-evolving complex fluorescence induction kinetics calcium lanthanides terbium.
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 9.Ghanotakis, D.F., Babcock, G.T., and Yocum, C.F., Structure of the oxygen-evolving complex of Photosystem II: Calcium and lanthanum compete for sites on the oxidizing side of Photosystem II which control the binding of water-soluble polypeptides and regulate the activity of the manganese complex, Biochim. Biophys. Acta, 1985, vol. 809, no. 2, pp. 173–180.CrossRefGoogle Scholar
- 11.Porra, R.J., Thompson, W.A., and Kriedemann, P.E., Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy, Biochim. Biophys. Acta, 1989, vol. 975, no. 3, pp. 384–394.CrossRefGoogle Scholar
- 13.Strasser, R.J. and Govindjee, On the O-J-I-P fluorescence transients in leaves and D1 mutants of Chlamydomonas reinhardtii, in Research in Photosynthesis, Murata, M., Ed., Dordrecht: Kluwer Academic Publ., 1992, vol. 2, pp. 29–32.Google Scholar
- 17.Loktyushkin, A.V., Lovyagina, E.R., and Semin, B.K., Inhibition of the electron-transport chain of the photosystem II with terbium cations, Akt. Vopr. Biol. Fiz. Khim., 2018, vol. 3, no. 2, pp. 250–255.Google Scholar
- 21.Semin, K., Davletshina, L.N., Timofeev, K.N., Ivanov, I.I., Rubin, A.B., and Seibert, M., Production of reactive oxygen species in decoupled, Ca2+-depleted PSII and their use in assigning a function to chloride on both sides of PSII, Photosynth. Res., 2013, vol. 117, nos. 1–3, pp. 385–399.CrossRefGoogle Scholar