Advertisement

A paradoxical effect of hydrated C60-fullerene at an ultralow concentration on the viability and aging of cultured Chinese hamster cells

  • O. I. Yablonskaya
  • T. S. Ryndina
  • V. L. Voeikov
  • A. N. Khokhlov
Gerontology

Abstract

The effect of an aqueous solution of hydrated C60-fullerene (HyFn) on the growth and “stationary phase aging” (accumulation of “age-related” changes in cultured cells during the slowing down of their proliferation within a single passage and the subsequent “aging” in the stationary phase of growth) of transformed B11-dii FAF28 Chinese hamster cells was studied. The final calculated concentration of HyFn in the growth medium was 10−19 M. A paradoxical result contrasting the available data on the absence of HyFn cytotoxicity at higher concentrations was obtained in our experiments: namely, HyFn decelerated cell proliferation (estimated by the growth of mass culture, as well as by the efficiency of colony formation) and accelerated the “stationary phase aging” of the cell culture. Moreover, repeated addition of an aqueous solution of HyFn (to the final calculated concentration of 10−19 M) to the cells that had already reached the stationary phase of growth caused a rapid (within no more than 24 h) death of a significant part of the cell population. The observed effect of HyFn at ultralow concentration is supposed to arise from the alterations in the properties of the water surrounding the fullerene molecule: namely, water becomes a donor and acceptor of electrons and regulates redox processes (especially those involving oxygen) in aqueous systems. This effect of HyFn at an ultralow concentration may be specific for transformed cells, and, therefore, experiments on normal fibroblasts with limited mitotic potential are planned as a continuation of the present study. It is also possible that the reported antiaging effect of HyFn in experimental animals is due to its anticancer, immunostimulatory, antiviral, and antibacterial properties manifested only at the whole-organism level.

Keywords

hydrated C60-fullerene ultralow concentrations cell cultures viability “stationary phase aging” colony-forming efficiency 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., Are Fullerenes Soluble in Water?, in Recent Advances in the Chemistry and Physics of Fullerenes and Related Materials, Ruoff, R.S. and Kadish, K.M., Eds., Reno: Electrochem. Soc. Inc., 1995, pp. 1591–1602.Google Scholar
  2. 2.
    Andrievsky, G.V., Klochkov, V.K., Bordyuh, A., and Dovbeshko, G.I., Comparative Analysis of Two Aqueous-Colloidal Solutions of C-60 Fullerene with Help of FTIP Reflectance and UV-Vis Spectroscopy, Chem. Phys. Lett., 2002, vol. 364, no. 1, pp. 8–17.CrossRefGoogle Scholar
  3. 3.
    Andrievsky, G.V., Klochkov, V.K., and Derevyanchenko, L.I., Is C60 Fullerene Molecule Toxic?!, Fullerenes, Nanotubes and Carbon Nanostructures, 2005, vol. 13, no. 4, pp. 363–376.CrossRefGoogle Scholar
  4. 4.
    Kolosnjaj, J., Szwarc, H., and Moussa, F., Toxicity Studies of Fullerenes and Derivatives, Adv. Exp. Med. Biol., 2007, vol. 620, pp. 168–180.PubMedCrossRefGoogle Scholar
  5. 5.
    Bakry, R. Vallant, R.M., Najam-Ul-Haq, M., Rainer, M., Szabo, Z., Huck, C.W., and Bonn, G.K., Medicinal Applications of Fullerenes, Int. J. Nanomed., 2007, vol. 2, no. 4, pp. 639–649.Google Scholar
  6. 6.
    Baati, T., Bourasset, F., Gharbi, N., Njim, L., Abderrabba, M., Kerkeni, A., Szwarc, H., and Moussa, F., The Prolongation of the Lifespan of Rats by Repeated Oral Administration of [60]Fullerene, Biomaterials, 2012, vol. 33, no. 19, pp. 4936–4946.PubMedCrossRefGoogle Scholar
  7. 7.
    Quick, K.L., Ali, S.S., Arch, R., Xiong, C., Wozniak, D., and Dugan, L.L., A Carboxyfullerene SOD Mimetic Improves Cognition and Extends the Lifespan of Mice, Neurobiol. Aging, 2008, vol. 29, no. 1, pp. 117–128.PubMedCrossRefGoogle Scholar
  8. 8.
    Levi, N., Hantgan, R.R., Lively, M.O., Carroll, D.L., and Prasad, G.L., C60-Fullerenes: Detection of Intracellular Photoluminescence and Lack of Cytotoxic Effects, J. Nanobiotechnol., 2006, vol. 4, no. 14, pp. 14–25.CrossRefGoogle Scholar
  9. 9.
    Mroz, P., Pawlak, A., Satti, M., Lee, H., Wharton, T., Gali, H., Sarna, T., and Hamblin, M.R., Functionalized Fullerenes Mediate Photodynamic Killing of Cancer Cells: Type I Versus Type II Photochemical Mechanism, Free Radic. Biol. Med., 2007, vol. 43, no. 5, pp. 711–719.PubMedCrossRefGoogle Scholar
  10. 10.
    Prylutska, S.V., Grynyuk, I.I., Palivoda, K.O., and Matyshevska, O.P., Photoinduced Cytotoxic Effect of Fullerenes C60 on Transformed T-Lymphocytes, Exp. Oncol., 2010, vol. 32, no. 1, pp. 29–32.PubMedGoogle Scholar
  11. 11.
    Ali, S.S., Hardt, J.I., Quick, K.L., Kim-Han, J.S., Erlanger, B.F., Huang, T.T., Epstein, C.J., and Dugan, L.L., A Biologically Effective Fullerene (C60) Derivative with Superoxide Dismutase Mimetic Properties, Free Radic. Biol. Med., 2004, vol. 37, no. 8, pp. 1191–1202.PubMedCrossRefGoogle Scholar
  12. 12.
    Andrievsky, G., Shakhnin, D., Tronza, A., Zhernosekov, D., and Tykhomyrov, A., The Acceleration of Blood Plasma Clot Lysis in the Presence of Hydrated C60 Fullerene Nanostructures in Super-Small Concentration, Fullerenes, Nanotubes and Carbon Nanostructures, 2010, vol. 18, no. 3, pp. 303–311.CrossRefGoogle Scholar
  13. 13.
    Andrievsky, G.V., Bruskov, V.I., Tykhomyrov, A.A., and Gudkov, S.V., Peculiarities of the Antioxidant and Radioprotective Effects of Hydrated C60 Fullerene Nanostructures in vitro and in vivo, Free Radic. Biol. Med., 2009, vol. 47, no. 6, pp. 786–793.PubMedCrossRefGoogle Scholar
  14. 14.
    Yablonskaya, O.I., Voeikov, V.L., Vilenskaya, N.D., Malishenko, S.I., and Novikov, K.N., Effects of Hydrated Fullerenes on the Luminescence of Bacterial Luciferase, of Whole Blood and of Bicarbonate Water Solutions, Luminescence, 2012, vol. 27, no. 2, p. 175.Google Scholar
  15. 15.
    Chirkova, E.Yu., Golovina, M.E., Nadzharyan, T.L., and Khokhlov, A.N., Cellular Kinetic Model for Studying Geroprotectors and Geropromoters, Dokl. Akad. Nauk SSSR, 1984, vol. 278, no. 6, pp. 1474–1476.PubMedGoogle Scholar
  16. 16.
    Khokhlov, A.N., Cell Proliferation and Aging, Itogi nauki i tekhniki VINITI AN SSSR. Ser. “Obshchie problemy fiziko-khimicheskoi biologii” (Advances in Science and Technology, Series General Problems of Physicochemical Biology), Moscow: VINITI, 1988, vol. 9.Google Scholar
  17. 17.
    Khokhlov, A.N., Prokhorov, L.Yu., Ivanov, A.S., and Archakov, A.I., Effects of Cholesterol- or 7-Ketocholesterol-Containing Liposomes on Colony-Forming Ability of Cultured Cells, FEBS Lett., 1991, vol. 290, nos. 1–2, pp. 171–172.PubMedCrossRefGoogle Scholar
  18. 18.
    Khokhlov, A.N., The Cell Kinetics Model for Determination of Organism Biological Age and for Geroprotectors or Geropromoters Studies, in Biomarkers of Aging: Expression and Regulation. Proceeding, Licastro, F. and Caldarera, C.M., Eds., Bologna: Clueb, 1992, pp. 209–216.Google Scholar
  19. 19.
    Khokhlov, A.N., Stationary Cell Cultures as a Tool for Gerontological Studies, Ann. N. Y. Acad. Sci., 1992, vol. 663, pp. 475–476.PubMedCrossRefGoogle Scholar
  20. 20.
    Khokhlov, A.N., Cytogerontology at the Beginning of the Third Millennium: From “Correlative” to “Gist” Models, Russ. J. Dev. Biol., 2003, vol. 34, no. 5, pp. 321–326.CrossRefGoogle Scholar
  21. 21.
    Khokhlov, A.N., In Search of “Gist” Cytogerontological Models, in Longevity, Aging and Degradation Models in Reliability, Public Health, Medicine and Biology, Antonov, V., Huber, C., Nikulin, M., and Polischook, V., Eds., St. Petersburg: SPbSPU, 2004, vol. 1, pp. 84–92.Google Scholar
  22. 22.
    Khokhlov, A.N., From Carrel to Hayflick and Back, or What We Got from the 100-Year Cytogerontological Studies, Biophysics, 2010, vol. 55, no. 5, pp. 859–864.CrossRefGoogle Scholar
  23. 23.
    Khokhlov, A.N., Does Aging Need an Own Program or the Existing Development Program Is More Than Enough?, Russ. J. Gen. Chem., 2010, vol. 80, no. 7, pp. 1507–1513.CrossRefGoogle Scholar
  24. 24.
    Alinkina, E.S., Vorobyova, A.K., Misharina, T.A., Fatkullina, L.D., Burlakova, E.B., and Khokhlov, A.N., Cytogerontological Studies of Biological Activity of Oregano Essential Oil, Mosc. Univ. Biol. Sci. Bull., 2012, vol. 67, no. 2, pp. 52–57.CrossRefGoogle Scholar
  25. 25.
    Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., On the Production of an Aqueous Colloidal Solution of Fullerenes, J. Chem. Soc., Chem. Commun., 1995, no. 12, pp. 1281–1282.Google Scholar

Copyright information

© Allerton Press, Inc. 2013

Authors and Affiliations

  • O. I. Yablonskaya
    • 1
  • T. S. Ryndina
    • 1
  • V. L. Voeikov
    • 1
  • A. N. Khokhlov
    • 1
  1. 1.School of BiologyMoscow State UniversityMoscowRussia

Personalised recommendations