A paradoxical effect of hydrated C60-fullerene at an ultralow concentration on the viability and aging of cultured Chinese hamster cells
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The effect of an aqueous solution of hydrated C60-fullerene (HyFn) on the growth and “stationary phase aging” (accumulation of “age-related” changes in cultured cells during the slowing down of their proliferation within a single passage and the subsequent “aging” in the stationary phase of growth) of transformed B11-dii FAF28 Chinese hamster cells was studied. The final calculated concentration of HyFn in the growth medium was 10−19 M. A paradoxical result contrasting the available data on the absence of HyFn cytotoxicity at higher concentrations was obtained in our experiments: namely, HyFn decelerated cell proliferation (estimated by the growth of mass culture, as well as by the efficiency of colony formation) and accelerated the “stationary phase aging” of the cell culture. Moreover, repeated addition of an aqueous solution of HyFn (to the final calculated concentration of 10−19 M) to the cells that had already reached the stationary phase of growth caused a rapid (within no more than 24 h) death of a significant part of the cell population. The observed effect of HyFn at ultralow concentration is supposed to arise from the alterations in the properties of the water surrounding the fullerene molecule: namely, water becomes a donor and acceptor of electrons and regulates redox processes (especially those involving oxygen) in aqueous systems. This effect of HyFn at an ultralow concentration may be specific for transformed cells, and, therefore, experiments on normal fibroblasts with limited mitotic potential are planned as a continuation of the present study. It is also possible that the reported antiaging effect of HyFn in experimental animals is due to its anticancer, immunostimulatory, antiviral, and antibacterial properties manifested only at the whole-organism level.
Keywordshydrated C60-fullerene ultralow concentrations cell cultures viability “stationary phase aging” colony-forming efficiency
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- 1.Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., Are Fullerenes Soluble in Water?, in Recent Advances in the Chemistry and Physics of Fullerenes and Related Materials, Ruoff, R.S. and Kadish, K.M., Eds., Reno: Electrochem. Soc. Inc., 1995, pp. 1591–1602.Google Scholar
- 5.Bakry, R. Vallant, R.M., Najam-Ul-Haq, M., Rainer, M., Szabo, Z., Huck, C.W., and Bonn, G.K., Medicinal Applications of Fullerenes, Int. J. Nanomed., 2007, vol. 2, no. 4, pp. 639–649.Google Scholar
- 9.Mroz, P., Pawlak, A., Satti, M., Lee, H., Wharton, T., Gali, H., Sarna, T., and Hamblin, M.R., Functionalized Fullerenes Mediate Photodynamic Killing of Cancer Cells: Type I Versus Type II Photochemical Mechanism, Free Radic. Biol. Med., 2007, vol. 43, no. 5, pp. 711–719.PubMedCrossRefGoogle Scholar
- 12.Andrievsky, G., Shakhnin, D., Tronza, A., Zhernosekov, D., and Tykhomyrov, A., The Acceleration of Blood Plasma Clot Lysis in the Presence of Hydrated C60 Fullerene Nanostructures in Super-Small Concentration, Fullerenes, Nanotubes and Carbon Nanostructures, 2010, vol. 18, no. 3, pp. 303–311.CrossRefGoogle Scholar
- 14.Yablonskaya, O.I., Voeikov, V.L., Vilenskaya, N.D., Malishenko, S.I., and Novikov, K.N., Effects of Hydrated Fullerenes on the Luminescence of Bacterial Luciferase, of Whole Blood and of Bicarbonate Water Solutions, Luminescence, 2012, vol. 27, no. 2, p. 175.Google Scholar
- 16.Khokhlov, A.N., Cell Proliferation and Aging, Itogi nauki i tekhniki VINITI AN SSSR. Ser. “Obshchie problemy fiziko-khimicheskoi biologii” (Advances in Science and Technology, Series General Problems of Physicochemical Biology), Moscow: VINITI, 1988, vol. 9.Google Scholar
- 18.Khokhlov, A.N., The Cell Kinetics Model for Determination of Organism Biological Age and for Geroprotectors or Geropromoters Studies, in Biomarkers of Aging: Expression and Regulation. Proceeding, Licastro, F. and Caldarera, C.M., Eds., Bologna: Clueb, 1992, pp. 209–216.Google Scholar
- 21.Khokhlov, A.N., In Search of “Gist” Cytogerontological Models, in Longevity, Aging and Degradation Models in Reliability, Public Health, Medicine and Biology, Antonov, V., Huber, C., Nikulin, M., and Polischook, V., Eds., St. Petersburg: SPbSPU, 2004, vol. 1, pp. 84–92.Google Scholar
- 25.Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., On the Production of an Aqueous Colloidal Solution of Fullerenes, J. Chem. Soc., Chem. Commun., 1995, no. 12, pp. 1281–1282.Google Scholar